Repeatedly usable immobilized pH gradient in a monolithic capillary column
文献类型：期刊论文
| 作者 | Yang, C; Zhu, GJ; Zhang, LH; Zhang, WB; Zhang, YK |
| 刊名 | electrophoresis
 |
| 出版日期 | 2004-06-01 |
| 卷号 | 25期号:12页码:1729-1734 |
| 关键词 | immobilized pH gradient isoelectric focusing monolithic column |
| 产权排序 | 1;1 |
| 英文摘要 | glycidyl methacrylate (gma) and ethylene dimethacrylate (edma) were used to synthesize a monolithic capillary column containing reactive epoxy groups. glutaraldehyde was introduced and linked to the monolith after a process of amination. an aqueous solution of commercial carrier ampholytes (cas, ampholine) was focused in such a polymer column. the primary amino groups of cas reacted with glutaraldehyde along the capillary. cas were immobilized at different positions in the column according to their isoelectric points (pl), resulting in a monolithic immobilized ph gradient (m-ipg). isoelectric focusing (ief) was performed without cas in such an m-ipg column. due to the covalent attachment of the cas this m-ipg can be repeatedly used after its preparation. good stability, linearity, and reproducibility were obtained. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | mass-spectrometry ; liquid-chromatography ; gel-electrophoresis ; 2-dimensional electrophoresis ; electrospray ionization ; macroporous polymer ; carrier ampholytes ; proteomics ; separation ; electrochromatography |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000222396000002 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/81643]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dept Biotechnol, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, C,Zhu, GJ,Zhang, LH,et al. Repeatedly usable immobilized pH gradient in a monolithic capillary column[J]. electrophoresis,2004,25(12):1729-1734. |
| APA | Yang, C,Zhu, GJ,Zhang, LH,Zhang, WB,&Zhang, YK.(2004).Repeatedly usable immobilized pH gradient in a monolithic capillary column.electrophoresis,25(12),1729-1734. |
| MLA | Yang, C,et al."Repeatedly usable immobilized pH gradient in a monolithic capillary column".electrophoresis 25.12(2004):1729-1734. |
入库方式： OAI收割
来源：大连化学物理研究所
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