Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application
文献类型：期刊论文
| 作者 | Ye, ZQ; Tan, MQ; Wang, GL; Yuan, JL |
| 刊名 | talanta
 |
| 出版日期 | 2005-01-15 |
| 卷号 | 65期号:1页码:206-210 |
| 关键词 | functionalized terbium nanoparticles fluorescence alpha-fetoprotein time-resolved fluoroimmunoassay |
| 产权排序 | 1;1 |
| 通讯作者 | 袁景利 |
| 英文摘要 | silica-based functionalized terbium fluorescent nanoparticles were prepared, characterized and developed as a fluorescence probe for antibody labeling and time-resolved fluoroimmunoassay. the nanoparticles were prepared in a water-in-oil (w/o) microemulsion containing a strongly fluorescent tb3+ chelate. n,n.n-1,n-1-12,6-bis(3'-aminomethyl-1'-pyrazolyl)phenylpyridine] tetrakis(acetate)-tb3+ (bpta-tb3+), triton x-100, octanol, and cyclohexane by controlling copolymerization of tetraethyl orthosilicate (teos) and 3-[2-(2- aminoethylamino)-ethylamino]propyl-trimethoxysilane (aeps) with ammonia water. the characterizations by transmission electron microscopy and fluorometric quantum methods show that the nanoparticles are spherical and uniform in size, 45 +/- 3 nm in diameter, strongly fluorescent with fluorescence yield of 10% and a long fluorescence lifetime of 2.0 ms. the amino groups directly introduced to the nanoparticle's surface by using aeps in the preparation made the surface modification and bioconjugation of the nanoparticles easier. the nanoparticle-labeled anti-human alpha-fetoprotein antibody was prepared and used for time-resolved fluoroimmunoassay of (x-fetoprotein (afp) in human serum samples. the assay response is linear from 0.10 ng ml(-1) to about 100 ng ml(-1) with the detection limit of 0.10 ng ml(-1). the coefficient variations (cvs) of the method are less than 9.0%. and the recoveries are in the range of 84-98% for human serum sample measurements. (c) 2004 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | prostate-specific antigen ; alpha-fetoprotein ; ultrasensitive immunoassays ; immunofluorometric assay ; poly(ethylene glycol) ; bioanalytical assays ; chelate ; serum ; fluorometry ; technology |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000225381600030 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/81717]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, ZQ,Tan, MQ,Wang, GL,et al. Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application[J]. talanta,2005,65(1):206-210. |
| APA | Ye, ZQ,Tan, MQ,Wang, GL,&Yuan, JL.(2005).Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application.talanta,65(1),206-210. |
| MLA | Ye, ZQ,et al."Development of functionalized terbium fluorescent nanoparticles for antibody labeling and time-resolved fluoroimmunoassay application".talanta 65.1(2005):206-210. |
入库方式： OAI收割
来源：大连化学物理研究所
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