Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry
文献类型：期刊论文
| 作者 | Xu, SY; Pan, CS; Hu, LG; Zhang, Y; Guo, Z; Li, X; Zou, HF |
| 刊名 | electrophoresis
 |
| 出版日期 | 2004-11-01 |
| 卷号 | 25期号:21-22页码:3669-3676 |
| 关键词 | desorption/ionization on silicon enzyme inhibitor immobilized enzyme matrix assisted laser desorption/ioniozation-time of flight-mass spectrometry miniaturization peptide mapping |
| 产权排序 | 1;1 |
| 英文摘要 | desorption/ionization on silicon mass spectrometry (dios-ms) is a matrix-free technique that allows for the direct desorption/ionization of low-molecular-weight compounds with little or no fragmentation of analytes. this technique has a relatively high tolerance for contaminants commonly found in biological samples. dios-ms has been applied to determine the activity of immobilized enzymes on the porous silicon surface. enzyme activities were also monitored with the addition of a competitive inhibitor in the substrate solution. it is demonstrated that this method can be applied to the screening of enzyme inhibitors. furthermore, a method for peptide mapping analysis by in situ digestion of proteins on the porous silicon surface modified by trypsin, combined with matrix-assisted laser desorption/ionization-time of flight-ms has been developed. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | protein identification ; peptide ; proteomics ; platform |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000225679000016 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/81995]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Chinese Acad Sci, Ecoenvironm Sci Res Ctr, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, SY,Pan, CS,Hu, LG,et al. Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry[J]. electrophoresis,2004,25(21-22):3669-3676. |
| APA | Xu, SY.,Pan, CS.,Hu, LG.,Zhang, Y.,Guo, Z.,...&Zou, HF.(2004).Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry.electrophoresis,25(21-22),3669-3676. |
| MLA | Xu, SY,et al."Enzymatic reaction of the immobilized enzyme on porous silicon studied by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry".electrophoresis 25.21-22(2004):3669-3676. |
入库方式： OAI收割
来源：大连化学物理研究所
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