Study on conical columns for semi-preparative liquid chromatography
文献类型：期刊论文
| 作者 | Ma, JP; Guan, YF; Chen, LX |
| 刊名 | journal of separation science
 |
| 出版日期 | 2003-03-01 |
| 卷号 | 26期号:3-4页码:307-312 |
| 关键词 | conical column flow profile zone shape semi-preparative liquid chromatography |
| 产权排序 | 1;1 |
| 英文摘要 | the dynamic flow profiles and column efficiencies in conically shaped semi-preparative liquid chromatographic columns (inlet id larger than outlet id) with two different conical angles (7degrees and 15degrees) were studied. the dynamic flow profiles were studied by an on-column visualization method. conical columns were compared with cylindrical column of the same length and internal volume. the results showed that the flow profile of a sample band in the conical column of 7degrees (50 mm x 17 mm --> 11 mm id) was parabolic in shape. the sample band migrated slower in the wall region than in the central region, as in the cylindrical column (50 mm x 14 mm id). however, the sample band in the conical column of 15degrees (50 mm x 20 mm --> 7 mm id) migrated slower in the central region than in the wall region, resulting in a reverse parabolic flow profile, in contrast to that in cylindrical column. this indicated that a flat flow profile might be realized in a conical column with a conical angle between 7degrees and 15degrees. the conical column of 15degrees had the highest column efficiency among the three columns under the same conditions. compared with the cylindrical column packed with identical packing material, the conical column of 15degrees had 22%-45% higher column efficiency and 11%-27% higher peak height. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | solute migration ; visualization ; diameter |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000181950200014 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/82153]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Analyt Chem & Micro Instrumentat, Dalian 116012, Peoples R China 2.Liaoning Univ Petr & Chem Technol, Dept Appl Chem, Fushun 113001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, JP,Guan, YF,Chen, LX. Study on conical columns for semi-preparative liquid chromatography[J]. journal of separation science,2003,26(3-4):307-312. |
| APA | Ma, JP,Guan, YF,&Chen, LX.(2003).Study on conical columns for semi-preparative liquid chromatography.journal of separation science,26(3-4),307-312. |
| MLA | Ma, JP,et al."Study on conical columns for semi-preparative liquid chromatography".journal of separation science 26.3-4(2003):307-312. |
入库方式： OAI收割
来源：大连化学物理研究所
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