Unilamellar liposomes covalently coupled on silica gel for liquid chromatography
文献类型：期刊论文
| 作者 | Mao, XQ; Kong, L; Li, X; Guo, BC; Zou, HF |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2003-02-01 |
| 卷号 | 375期号:4页码:550-555 |
| 关键词 | covalent coupling liposome silica gel peptides Chinese medicine |
| 产权排序 | 1;1 |
| 英文摘要 | silica gel was used as a support for the covalent coupling of liposomes, which could overcome drawbacks of soft gel beads in column efficiency and separation speed. the influences of the concentration of added dimethylaminopyridine and reaction time on the chloroformate activation reaction of silica gel were investigated. temperature and ph for covalent coupling of liposomes on the activated silica gel were also optimized. experimental results indicated that the stability of the covalently coupled liposome columns was obviously superior to that of the noncovalently coated liposome columns but the selectivity of both columns was basically identical. separation and analysis of a crude extract of a traditional chinese medicine ligusticum wallichii and a mixture of small peptides on both columns further support this conclusion. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | immobilized-liposome ; biomembrane chromatography ; membrane interactions ; drugs ; proteins ; beads ; prediction ; column |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000181659600014 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/82391]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mao, XQ,Kong, L,Li, X,et al. Unilamellar liposomes covalently coupled on silica gel for liquid chromatography[J]. analytical and bioanalytical chemistry,2003,375(4):550-555. |
| APA | Mao, XQ,Kong, L,Li, X,Guo, BC,&Zou, HF.(2003).Unilamellar liposomes covalently coupled on silica gel for liquid chromatography.analytical and bioanalytical chemistry,375(4),550-555. |
| MLA | Mao, XQ,et al."Unilamellar liposomes covalently coupled on silica gel for liquid chromatography".analytical and bioanalytical chemistry 375.4(2003):550-555. |
入库方式： OAI收割
来源：大连化学物理研究所
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