Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry
文献类型：期刊论文
| 作者 | Wang ZH(王战会); Jin G(靳刚)
|
| 刊名 | Journal of Immunological Methods
 |
| 出版日期 | 2004 |
| 卷号 | 285期号:2页码:237-243 |
| 通讯作者邮箱 | gajin@imech.ac.cn |
| ISSN号 | 0022-1759 |
| 通讯作者 | Jin, G (reprint author), Chinese Acad Sci, Inst Mech, Natl Micrograv Lab, Beijing 100080, Peoples R China. |
| 中文摘要 | In the development of biosensors, the immobilization of biomolecules at interfaces played a crucial role. The feasibility of using 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde (Glu) to modify silicon surface to immobilize covalently protein for immunoassay with the biosensor based on imaging ellipsometry was investigated. The higher density and stability of human IgG layer could be obtained on the silicon surface modified with APTES and Glu than that on the silicon surface modified with dichlorodimethylsilane (DIDS). The human IgG molecules immobilized covalently on APTES-Glu surface bound more anti-IgG molecules than that on DDS surface, which indicated that the human IgG molecules could maintain higher binding capability on APTES-Glu surface. Tween 20 was able to block the undesirable adsorption on APTES-Glu surface, and also enhanced the recognition between human IgG and its antibody on both APTES-Glu and DDS surfaces. The combination of this protein covalent immobilization and the biosensor has the potential to be developed into a fast, simple immunoassay technique. |
| 学科主题 | 力学 |
| 类目[WOS] | Biochemical Research Methods ; Immunology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Immunology |
| 关键词[WOS] | SILICA SURFACES ; VISUALIZATION ; ANTIBODY ; INTERFACES ; PLASMA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000220140200009 |
| 公开日期 | 2007-06-15 ; 2007-12-05 ; 2009-06-23 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/15439]  |
| 专题 | 力学研究所_力学所知识产出(1956-2008) |
| 推荐引用方式 GB/T 7714 | Wang ZH,Jin G. Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry[J]. Journal of Immunological Methods,2004,285(2):237-243. |
| APA | 王战会,&靳刚.(2004).Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry.Journal of Immunological Methods,285(2),237-243. |
| MLA | 王战会,et al."Covalent Immobilization of Proteins for the Biosensor Based on Imaging Ellipsometry".Journal of Immunological Methods 285.2(2004):237-243. |
入库方式： OAI收割
来源：力学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。