Analysis of specific gene by integration of isothermal amplification and electrophoresis on poly(methyl methacrylate) microchips
文献类型：期刊论文
| 作者 | Hataoka, Y; Zhang, LH; Mori, Y; Tomita, N; Notomi, T; Baba, Y |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2004-07-01 |
| 卷号 | 76期号:13页码:3689-3693 |
| 通讯作者 | 张丽华 |
| 英文摘要 | we have successfully achieved the integration of isothermal amplification and the subsequent analysis of specific gene fragments on poly(methyl methacrylate) microchips. in our experiments, loop-mediated isothermal amplification, which can offer higher specificity and efficiency than pcr, has been performed at a constant temperature (65 degreesc). after amplification, products could be either examined by the integrated microchip-based electrophoresis or directly observed by naked eye with sybr green i added into the reaction solution. by such an integrated microsystem, the amplification and the subsequent analysis of prostate-specific antigen gene with template concentration at 23 fg/mul could be finished within 15 min, which demonstrates its advantages of high specificity, good reproducibility, and fast speed in gene detection. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | dna amplification ; device ; separation ; systems |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000222418300037 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/82565]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Tokushima, Grad Sch Pharmaceut Sci, Dept Mol & Phamaceut Biotechnol, CREST,JST, Tokushima 7708505, Japan 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 3.Natl Inst Adv Ind Sci & Technol, AIST, Single Mol Bioanal Lab, Takamatsu, Kagawa 7610395, Japan |
| 推荐引用方式 GB/T 7714 | Hataoka, Y,Zhang, LH,Mori, Y,et al. Analysis of specific gene by integration of isothermal amplification and electrophoresis on poly(methyl methacrylate) microchips[J]. analytical chemistry,2004,76(13):3689-3693. |
| APA | Hataoka, Y,Zhang, LH,Mori, Y,Tomita, N,Notomi, T,&Baba, Y.(2004).Analysis of specific gene by integration of isothermal amplification and electrophoresis on poly(methyl methacrylate) microchips.analytical chemistry,76(13),3689-3693. |
| MLA | Hataoka, Y,et al."Analysis of specific gene by integration of isothermal amplification and electrophoresis on poly(methyl methacrylate) microchips".analytical chemistry 76.13(2004):3689-3693. |
入库方式： OAI收割
来源：大连化学物理研究所
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