Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers
文献类型：期刊论文
| 作者 | Zhao, QY; Jin, MF; Muller, WEG; Zhang, W; Yu, XJ; Deng, MC |
| 刊名 | biotechnology progress
 |
| 出版日期 | 2003-09-01 |
| 卷号 | 19期号:5页码:1569-1573 |
| 产权排序 | 1;1 |
| 英文摘要 | toward the development of an in vitro cultivation of marine sponge cells for sustainable production of bioactive metabolites, the attachment characteristics of marine sponge cells of hymeniacidon perleve on three types of microcarriers, hillex, cytodex 3, and glass beads, were studied. mixed cell population and enriched cell fractions of specific cell types by ficoll gradient centrifugation (6%/8%/15%/20%) were also assessed. cell attachment ratio (defined as the ratio of cells attached on microcarrier to the total number of cells in the culture) on glass beads is much higher than that on cytodex 3 and hillex for both mixed cell population and cell fraction at ficoll 15-20% interface. the highest attachment ratio of 41% was obtained for the cell fraction at ficoll 15-20% interface on glass beads, which was significantly higher than that of a mixed cell population (18%). the attachment kinetics on glass beads indicated that the attachment was completed within 1 h. cell attachment ratio decreases with increase in cell-to-microcarrier ratio (3-30 cells/bead) and ph (7.6-9.0). the addition of serum and bsa (bovine serum albumin) reduced the cell attachment on glass beads. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology ; food science & technology |
| 研究领域[WOS] | biotechnology & applied microbiology ; food science & technology |
| 关键词[WOS] | suberites-domuncula ; vero cells ; dysidea-avara ; primmorphs ; culture ; cultivation ; growth ; proteins ; kinetics ; origin |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000185816400024 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/82757]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Univ Mainz, Inst Physiol Chem, Angew Mol Biol Abt, D-55099 Mainz, Germany 3.Flinders Univ S Australia, Sch Med, CRC Bioprod, Bedford Pk, SA 5042, Australia 4.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia |
| 推荐引用方式 GB/T 7714 | Zhao, QY,Jin, MF,Muller, WEG,et al. Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers[J]. biotechnology progress,2003,19(5):1569-1573. |
| APA | Zhao, QY,Jin, MF,Muller, WEG,Zhang, W,Yu, XJ,&Deng, MC.(2003).Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers.biotechnology progress,19(5),1569-1573. |
| MLA | Zhao, QY,et al."Attachment of marine sponge cells of Hymeniacidon perleve on microcarriers".biotechnology progress 19.5(2003):1569-1573. |
入库方式： OAI收割
来源：大连化学物理研究所
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