Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis
文献类型：期刊论文
| 作者 | Zhang, XY; Cao, XP; Zhang, W; Yu, XJ; Jin, MF |
| 刊名 | biotechnology and bioengineering
 |
| 出版日期 | 2003-12-05 |
| 卷号 | 84期号:5页码:583-590 |
| 关键词 | porifera sponges primmorph archaeocytes cell culture spiculogenesis Hymeniacidon perleve |
| 产权排序 | 1;1 |
| 英文摘要 | marine sponges (porifera) possess an extraordinary diversity of bioactive metabolites for new drug discovery and development. in vitro cultivation of sponge cells in a bioreactor system is very attractive for the sustainable production of sponge-derived bioactive metabolites; however, it is still a challenging task. the recent establishment of sponge primmorphs, multicellular aggregates from dissociated mixed-cell population (mcp), has been widely acknowledged to hold great promise for cultivation in vitro. here we present a new method to establish an in vitro sponge primmorph culture from archaeocyte-dominant cell population (adcp) enriched by a ficoll gradient, rather than a mixed-cell population (mcp). our rationale is based upon the totipotency (the ability of a cell to differentiate into other cell types) of archaeocyte cells and the different biological functions of various sponge cell types. a sponge, hymeniacidon perleve collected from the china yellow sea was used as a model system for this investigation. distinct dynamics of primmorph formation were observed while significant increases in dna synthesis, cell proliferation (up to threefold), and cell growth (up to fourfold) were achieved. furthermore, a time-dependent spiculogenesis was clearly demonstrated in our longterm culture, indicating high metabolic activity of primmorphs from the adcp. this new method represents an important step forward to advance sponge cell culture in vitro that may lead to commercial exploitation of sponge-derived drugs. (c) 2003 wiley periodicals, inc. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | marine natural-products ; suberites-domuncula ; dysidea-avara ; in-vitro ; fractionation ; cultivation ; culture |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000186316800008 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/82781]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Div Biotechnol, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Flinders Univ S Australia, CRC Bioprod, Bedford Pk, SA 5042, Australia 3.Flinders Univ S Australia, Sch Med, Dept Med Biotechnol, Bedford Pk, SA 5042, Australia |
| 推荐引用方式 GB/T 7714 | Zhang, XY,Cao, XP,Zhang, W,et al. Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis[J]. biotechnology and bioengineering,2003,84(5):583-590. |
| APA | Zhang, XY,Cao, XP,Zhang, W,Yu, XJ,&Jin, MF.(2003).Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis.biotechnology and bioengineering,84(5),583-590. |
| MLA | Zhang, XY,et al."Primmorphs from archaeocytes-dominant cell population of the sponge Hymeniacidon perleve: Improved cell proliferation and spiculogenesis".biotechnology and bioengineering 84.5(2003):583-590. |
入库方式： OAI收割
来源：大连化学物理研究所
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