Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley)
文献类型：期刊论文
| 作者 | Zhang, W; Zhang, XY; Cao, XP; Xu, JY; Zhao, QY; Yu, XJ; Jin, MF; Deng, MC |
| 刊名 | journal of biotechnology
 |
| 出版日期 | 2003-01-23 |
| 卷号 | 100期号:2页码:161-168 |
| 关键词 | sponges primmorph in vitro cultivation Stylotella agminata optimization |
| 产权排序 | 1;1 |
| 英文摘要 | the establishment and optimization of in vitro primmorph formation from a chinese sponge, stylotella agminata (ridley), collected from the south china sea, were investigated. our aims were to identify the key factors affecting primmorph formation in this species and to optimize the technique for developing an in vitro primmorph culture system. the size of dissociated cells from s. agminata is relatively small, in the range between 5 and 10 mum. round-shaped primmorphs of less than 100 gm were formed 3 days after transferring the dissociated cells into seawater containing ca2+ and mg2+. the effect of various cell dissociation conditions, inoculum. cell density, concentration of antibiotics, ph, and temperature was further investigated upon the formation of primmorphs. the time required for primmorph formation, primmorph size distribution, and the proliferating capability were microscopically documented. healthy sponge s. agminata, inoculum. cell density and culture temperature play a critical role for the successful formation of primmorphs and that the microbial contamination will have to be controlled. (c) 2002 elsevier science b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | primary-cell culture ; suberites-domuncula |
| 收录类别 | ISTP ; SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000180446200008 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/82783]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, W,Zhang, XY,Cao, XP,et al. Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley)[J]. journal of biotechnology,2003,100(2):161-168. |
| APA | Zhang, W.,Zhang, XY.,Cao, XP.,Xu, JY.,Zhao, QY.,...&Deng, MC.(2003).Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley).journal of biotechnology,100(2),161-168. |
| MLA | Zhang, W,et al."Optimizing the formation of in vitro sponge primmorphs from the Chinese sponge Stylotella agminata (Ridley)".journal of biotechnology 100.2(2003):161-168. |
入库方式： OAI收割
来源：大连化学物理研究所
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