A single-strand conformation polymorphism method by capillary electrophoresis with laser-induced fluorescence for detection of the T1151A mutation in hMLH1 gene
文献类型：期刊论文
| 作者 | Shi, XZ; Xu, GW; Zhao, CX; Ma, JM; Zhang, Y; Lv, S; Yang, Q |
| 刊名 | electrophoresis
 |
| 出版日期 | 2003-07-01 |
| 卷号 | 24期号:14页码:2316-2321 |
| 关键词 | colorectal cancer hMLH1 gene lung cancer single-strand conformation polymorphism |
| 产权排序 | 1;1 |
| 英文摘要 | mutation of hmlh1 gene plays an important role in human tumorigenesis. a highly sensitive single-strand conformation polymorphism (sscp) method for detection of the t1151a mutation in exon 12 of the hmlh1 gene was for the first time developed employing laser-induced fluorescence capillary electrophoresis (lif-ce). effects of the concentration of linear polyacrylamide solution, running temperature, running voltage and the addition of glycerol on sscp analysis were investigated, and the optimum separation conditions were defined. thirty colorectal cancer patients and eight lung cancer patients were screened and the t1151a mutation was found in four of them. based on ce-sequencing the mutation was further confirmed. to our knowledge, this is for the first time that the t1151a mutation is found in lung cancer. our method is simple, rapid, and highly sensitive and is well suited to the analysis of large numbers of clinical samples. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | point mutations ; dna fragments ; mismatch repair ; high-throughput ; sieving medium ; receptor gene ; separation ; efficient ; allele |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000184525200008 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/83121]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, XZ,Xu, GW,Zhao, CX,et al. A single-strand conformation polymorphism method by capillary electrophoresis with laser-induced fluorescence for detection of the T1151A mutation in hMLH1 gene[J]. electrophoresis,2003,24(14):2316-2321. |
| APA | Shi, XZ.,Xu, GW.,Zhao, CX.,Ma, JM.,Zhang, Y.,...&Yang, Q.(2003).A single-strand conformation polymorphism method by capillary electrophoresis with laser-induced fluorescence for detection of the T1151A mutation in hMLH1 gene.electrophoresis,24(14),2316-2321. |
| MLA | Shi, XZ,et al."A single-strand conformation polymorphism method by capillary electrophoresis with laser-induced fluorescence for detection of the T1151A mutation in hMLH1 gene".electrophoresis 24.14(2003):2316-2321. |
入库方式： OAI收割
来源：大连化学物理研究所
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