Simultaneous genotyping of multiplex single nucleotide polymorphisms of the K-ras gene with a home-made kit
文献类型：期刊论文
| 作者 | Zhao, CX; Xu, GW; Shi, XZ; Ma, JM; Zhang, Y; Lv, S; Yang, Q |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2003-09-25 |
| 卷号 | 795期号:1页码:55-60 |
| 关键词 | K-ras gene single nucleotide polymorphism SNaPshot |
| 产权排序 | 1;1 |
| 英文摘要 | accurate and fast genotyping of single nucleotide polymorphisms (snps) is important in the human genome project. here an automated fluorescent method that can rapidly and accurately genotype multiplex known snps was developed by using a homemade kit, which has lower cost but higher resolution than commercial kit. with this method, oncogene k-ras was investigated, four known snps of k-ras gene exon 1 in 31 coloerctal cancer patients were detected. results indicate that mutations were present in 8(26%) of 31 patients, and most mutations were localized in codon 12. the presence of these mutations is thought to be a critical step and plays an important role in human colorectal carcinogenesisas. (c) 2003 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | strand conformation polymorphism ; linear polyacrylamide solutions ; sequencing extension products ; capillary-electrophoresis ; colorectal-cancer ; oncogenes ; mutations ; p53 ; separation ; bases |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000185270400007 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/83127]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Dalian Med Univ, Affiliated Hosp 2, Dalian 116027, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, CX,Xu, GW,Shi, XZ,et al. Simultaneous genotyping of multiplex single nucleotide polymorphisms of the K-ras gene with a home-made kit[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2003,795(1):55-60. |
| APA | Zhao, CX.,Xu, GW.,Shi, XZ.,Ma, JM.,Zhang, Y.,...&Yang, Q.(2003).Simultaneous genotyping of multiplex single nucleotide polymorphisms of the K-ras gene with a home-made kit.journal of chromatography b-analytical technologies in the biomedical and life sciences,795(1),55-60. |
| MLA | Zhao, CX,et al."Simultaneous genotyping of multiplex single nucleotide polymorphisms of the K-ras gene with a home-made kit".journal of chromatography b-analytical technologies in the biomedical and life sciences 795.1(2003):55-60. |
入库方式： OAI收割
来源：大连化学物理研究所
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