Study of competitive binding of enantiomers to protein by affinity capillary electrochromatography
文献类型：期刊论文
| 作者 | Ye, ML; Zou, HF; Liu, Z; Wu, RN; Lei, ZD; Ni, JY |
| 刊名 | journal of pharmaceutical and biomedical analysis
 |
| 出版日期 | 2002-01-15 |
| 卷号 | 27期号:3-4页码:651-660 |
| 关键词 | affinity capillary electrochromatography enantiomers drug-protein interaction bovine serum albumin |
| 英文摘要 | affinity capillary electrochromatography (cec) with zonal elution method was used to probe the competitive interactions of enantiomers with protein. in this approach, a known concentration of a competing agent is continuously applied to a cec column with bovine serum albumin (bsa) physically adsorbed on sax packing while injections of a small amount of analyte are made. the binding sites of solutes on the bsa molecule were determined by the changes in the retention factors of the solutes resulted from the addition of competitive agent. by using d- or l-tryptophan as competitive agents and d-, l-tryptophan and benzoin enantiomers as injected analytes showed that bsa molecule has a primary site to strongly bind l-tryptophan, but d-tryptophan dose not bind at this site; d- and l-tryptophan share a weak binding site on the bsa molecule. benzoin enantiomers do not share any binding sites with either d- or l-tryptophan. non-chiral compounds of trichloroacetic acid and n-hexanoic acid were applied as the competitive agents to study the binding of warfarin enantiomers to bsa, it was observed that trichloroacetic acid and n-hexanoic acid had a same binding site for warfarin enantiomers binding to bsa molecule. (c) 2002 elsevier science b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences ; life sciences & biomedicine |
| 类目[WOS] | chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | bovine serum-albumin ; chromatography ; separation ; technology ; tryptophan ; retention |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000173430500028 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/83223]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, ML,Zou, HF,Liu, Z,et al. Study of competitive binding of enantiomers to protein by affinity capillary electrochromatography[J]. journal of pharmaceutical and biomedical analysis,2002,27(3-4):651-660. |
| APA | Ye, ML,Zou, HF,Liu, Z,Wu, RN,Lei, ZD,&Ni, JY.(2002).Study of competitive binding of enantiomers to protein by affinity capillary electrochromatography.journal of pharmaceutical and biomedical analysis,27(3-4),651-660. |
| MLA | Ye, ML,et al."Study of competitive binding of enantiomers to protein by affinity capillary electrochromatography".journal of pharmaceutical and biomedical analysis 27.3-4(2002):651-660. |
入库方式： OAI收割
来源：大连化学物理研究所
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