Study on the multiple sites binding of human serum albumin and porphyrin by affinity capillary electrophoresis
文献类型：期刊论文
| 作者 | Zhang, WB; Zhang, LH; Ping, GC; Zhang, YK; Kettrup, A |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2002-02-25 |
| 卷号 | 768期号:1页码:211-214 |
| 关键词 | affinity capillary electrophoresis protein binding human serum albumin porphyrin |
| 英文摘要 | equations to describe the two sites binding between proteins and ligands were deduced. according to these equations, not only the binding constants, but also the mole fraction of proteins in different forms could be obtained. using the published data on the interaction between human serum albumin (hsa) and three kinds of porphyrin (coproporphyrin (cp), uroporphyrin i (up) and protoporphyrin (pp)), a further study on their binding was carried out. it was concluded that there may exist two binding sites with the binding constants at the first site. proved to be the preferential one, being 6.50 x 10(5) 1.94 x 10(6) and 8.94 x 10(5). respectively. in addition. it was also demonstrated that the two binding sites of hsa with cp and up might be of different kinds, though those of hsa and pp were of the same kind but at different positions. (c) 2002 elsevier science b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | chromatography ; thermodynamics ; hemopexin ; drug |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000173925700021 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/83291]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.GSF, Natl Res Ctr Environm & Hlth, Inst Ecol Chem, D-85764 Neuherberg, Germany |
| 推荐引用方式 GB/T 7714 | Zhang, WB,Zhang, LH,Ping, GC,et al. Study on the multiple sites binding of human serum albumin and porphyrin by affinity capillary electrophoresis[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2002,768(1):211-214. |
| APA | Zhang, WB,Zhang, LH,Ping, GC,Zhang, YK,&Kettrup, A.(2002).Study on the multiple sites binding of human serum albumin and porphyrin by affinity capillary electrophoresis.journal of chromatography b-analytical technologies in the biomedical and life sciences,768(1),211-214. |
| MLA | Zhang, WB,et al."Study on the multiple sites binding of human serum albumin and porphyrin by affinity capillary electrophoresis".journal of chromatography b-analytical technologies in the biomedical and life sciences 768.1(2002):211-214. |
入库方式： OAI收割
来源：大连化学物理研究所
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