Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix
文献类型：期刊论文
| 作者 | Wang, H; Qin, JH; Dai, ZP; Wang, L; Bai, JL; Lin, BC |
| 刊名 | journal of separation science
 |
| 出版日期 | 2003-07-01 |
| 卷号 | 26期号:9-10页码:869-874 |
| 关键词 | low viscosity DNA electrophoresis microchip |
| 产权排序 | 1;1 |
| 英文摘要 | a novel protocol has been established to separate dsdna fragments with high efficiency on glass chips by using an ultralow viscosity sieving matrix with added glucose. low-molecular-weight hydroxypropylmethylcellulose (hpmc), with a viscosity nearly equivalent to that of water, was used to electrophoretically separate fluorescent inter-calator-labeled double-stranded dna (dsdna) fragments on microfluidic glass chips. in comparison with conventional sieving protocols, low-molecular-weight hpmc as sieving matrix could result in reduced running cost and analysis time, in addition to a comparable separation efficiency of dsdna fragments. in this paper, the addition of glucose was investigated to enhance the separation of dna in the lowest viscosity polymer evaluated. the effect of staining dye and field strength were also evaluated. at an applied electric field strength of 200 v/cm, satisfactory resolution of the pbr322/haeiii dna marker could be achieved within 4 min by using 2% hpmc-5 with 6% glucose added. coelectrophoresing pcr product along with phix174/haeiii dna sizing marker was also demonstrated by using the ultralow viscosity hpmc-5 solution on a glass chip. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | entangled polymer-solutions ; tandem repeat loci ; capillary-electrophoresis ; dna separations ; mutations |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000184392100017 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/83395]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, H,Qin, JH,Dai, ZP,et al. Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix[J]. journal of separation science,2003,26(9-10):869-874. |
| APA | Wang, H,Qin, JH,Dai, ZP,Wang, L,Bai, JL,&Lin, BC.(2003).Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix.journal of separation science,26(9-10),869-874. |
| MLA | Wang, H,et al."Highly efficient separation of dsDNA fragments on glass chips by using an ultralow viscosity sieving matrix".journal of separation science 26.9-10(2003):869-874. |
入库方式： OAI收割
来源：大连化学物理研究所
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