Immobilized metal-ion chelating capillary microreactor for peptide mapping analysis of proteins by matrix assisted laser desorption/ionization-time of flight-mass spectrometry
文献类型：期刊论文
| 作者 | Guo, Z; Xu, SY; Lei, ZD; Zou, HF; Guo, BC |
| 刊名 | electrophoresis
 |
| 出版日期 | 2003-11-01 |
| 卷号 | 24期号:21页码:3633-3639 |
| 关键词 | capillary microreactor immobilized enzyme matrix assisted laser desorption/ionization-time of flight-mass spectrometry metal-ion chelated adsorption miniaturization peptide mapping analysis |
| 产权排序 | 1;1 |
| 英文摘要 | peptide mass mapping analysis, utilizing a regenerable enzyme microreactor with metal-ion chelated adsorption of enzyme, combined with matrix assisted laser desorption/ionization-time of flight-mass spectrometry (maldi-tof-ms) was developed. different procedures from the conventional approaches were adopted to immobilize the chelator onto the silica supports, that is, the metal chelating agent of iminodiacetic acid (ida) was reacted with glycidoxypropyltrimethoxysilane (glymo) before its immobilization onto the inner wall of the fused-silica capillary pretreated with nh4hf2. the metal ion of copper and subsequently enzyme was specifically adsorbed onto the surface to form the immobilized enzyme capillary microreactor, which was combined with maldi-tof-ms to apply for the mass mapping analysis of nl amounts of protein samples. the results revealed that the peptide mapping could routinely be generated from 0.5 pmol protein sample in 15 min at 50degreesc, even 20 fmol cytochrome c could be well digested and detected. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | trypsin ; electrophoresis ; identification ; databases ; reactor |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000186858700010 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/83443]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA |
| 推荐引用方式 GB/T 7714 | Guo, Z,Xu, SY,Lei, ZD,et al. Immobilized metal-ion chelating capillary microreactor for peptide mapping analysis of proteins by matrix assisted laser desorption/ionization-time of flight-mass spectrometry[J]. electrophoresis,2003,24(21):3633-3639. |
| APA | Guo, Z,Xu, SY,Lei, ZD,Zou, HF,&Guo, BC.(2003).Immobilized metal-ion chelating capillary microreactor for peptide mapping analysis of proteins by matrix assisted laser desorption/ionization-time of flight-mass spectrometry.electrophoresis,24(21),3633-3639. |
| MLA | Guo, Z,et al."Immobilized metal-ion chelating capillary microreactor for peptide mapping analysis of proteins by matrix assisted laser desorption/ionization-time of flight-mass spectrometry".electrophoresis 24.21(2003):3633-3639. |
入库方式： OAI收割
来源：大连化学物理研究所
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