Separation of peptides by pressurized capillary electrochromatography
文献类型：期刊论文
| 作者 | Zhang, K; Jiang, ZJ; Yao, CY; Zhang, ZC; Wang, QS; Gao, RY; Yan, C |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2003-02-14 |
| 卷号 | 987期号:1-2页码:453-458 |
| 关键词 | electrochromatography pressurized capillary electrochromatography gradient elution peptides |
| 产权排序 | 2;2 |
| 英文摘要 | a pressurized electrochromatography (pcec) instrument with gradient capability was used in this work for separation of peptides. three separation modes, namely, pcec, high-performance liquid chromatography and capillary electrophoresis can be carried out with the instrument. in pcec mode, the mobile phase is driven by both electroosmotic flow and pressurized flow, facilitating fine-tuning in selectivity of neutral and charged species. a continuous gradient elution can be carried out conveniently on this instrument, which demonstrates that it is more powerful than isocratic pcec for separation of complicated samples. the effects of applied voltage, supplementary pressure and ion-pairing agents on separation of peptides in gradient pcec were investigated. the effects of flow-rate of the pump and the volume of the mixer on resolution were also evaluated. (c) 2002 elsevier science b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | flight mass detector ; liquid-chromatography ; gradient elution ; bonded silica ; derivatives |
| 收录类别 | ISTP ; SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000180890500052 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/83485]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, State Key Lab Elemento Organ Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116011, Peoples R China 3.Unimicro Technol Inc, Pleasanton, CA 94566 USA |
| 推荐引用方式 GB/T 7714 | Zhang, K,Jiang, ZJ,Yao, CY,et al. Separation of peptides by pressurized capillary electrochromatography[J]. journal of chromatography a,2003,987(1-2):453-458. |
| APA | Zhang, K.,Jiang, ZJ.,Yao, CY.,Zhang, ZC.,Wang, QS.,...&Yan, C.(2003).Separation of peptides by pressurized capillary electrochromatography.journal of chromatography a,987(1-2),453-458. |
| MLA | Zhang, K,et al."Separation of peptides by pressurized capillary electrochromatography".journal of chromatography a 987.1-2(2003):453-458. |
入库方式： OAI收割
来源：大连化学物理研究所
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