Pressurized capillary electrochromatography separation of peptides with strong cation exchange and hydrophilic interaction
文献类型：期刊论文
| 作者 | Zhang, K; Gao, RY; Jiang, ZJ; Yao, CY; Zhang, ZC; Wang, QS; Yan, C |
| 刊名 | journal of separation science
 |
| 出版日期 | 2003-10-01 |
| 卷号 | 26期号:15-16页码:1389-1394 |
| 关键词 | capillary electrochromatography (CEC) pressurized capillary electrochromatography (pCEC) hydrophilic chromatography peptides |
| 产权排序 | 2;7 |
| 英文摘要 | a pressurized capillary electrochromatography (pcec) instrument with solvent gradient capability has been used for the separation of a peptide mixture. retention mechanism and selectivity of the peptides were studied by pcec using a strong cation exchange (scx) column. the effects of applied voltage, supplementary pressure, organic modifier concentration, ionic strength,, and ph value on pcec separation were investigated. it was found that the retention mechanism of the peptides in this system is based on a mixed mode of hydrophilic interaction, strong cation exchange, and electrophoresis. compared with the separation results obtained by reverse phase pcec and capillary electrophoresis (ce), this mixed-mode pcec is more powerful for the separation of hydrophilic peptides with similar charge-to-mass ratio. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | liquid-chromatography ; bonded silica ; columns ; electrophoresis ; derivatives ; retention ; proteins ; detector ; phases ; flow |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000186375800011 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/83491]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, State Key Lab Elemento Organ Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dept Biotechnol, Dalian 116011, Peoples R China 3.Unimicro Technol Inc, Pleasanton, CA 94566 USA |
| 推荐引用方式 GB/T 7714 | Zhang, K,Gao, RY,Jiang, ZJ,et al. Pressurized capillary electrochromatography separation of peptides with strong cation exchange and hydrophilic interaction[J]. journal of separation science,2003,26(15-16):1389-1394. |
| APA | Zhang, K.,Gao, RY.,Jiang, ZJ.,Yao, CY.,Zhang, ZC.,...&Yan, C.(2003).Pressurized capillary electrochromatography separation of peptides with strong cation exchange and hydrophilic interaction.journal of separation science,26(15-16),1389-1394. |
| MLA | Zhang, K,et al."Pressurized capillary electrochromatography separation of peptides with strong cation exchange and hydrophilic interaction".journal of separation science 26.15-16(2003):1389-1394. |
入库方式： OAI收割
来源：大连化学物理研究所
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