Binding of metal ions with protein studied by a combined technique of microdialysis with liquid chromatography
文献类型：期刊论文
| 作者 | Guo, M; Zou, HF; Wang, HL; Kong, L; Ni, JY |
| 刊名 | analytica chimica acta
 |
| 出版日期 | 2001-09-12 |
| 卷号 | 443期号:1页码:91-99 |
| 关键词 | binding interaction metal ions human serum albumin microdialysis HPLC |
| 英文摘要 | a method has been developed for the determination of interactions of metal ions and protein by using microdialysis sampling technique combined with pre-column derivation and reversed-phase ion-pair liquid chromatographic (hplc analysis. cu(ii), zn(ii) and human serum albumin (hsa) were chosen as model metal ions and protein, respectively. the mixed solutions of metal ions and hsa with different molar ratios buffered with 0.1 m tris-hcl containing 0.1 m nacl at ph 7.43 were sampled with a mirodialysis probe by keeping perfusion rate at 1 mul/min and the temperature at 37 degreesc. the free concentrations of metal ions in microdialysates were assayed by precolumn derivatization with meso-tetra(4-sulfophenyl)-porphyrin (tpps4) followed ion-pair hplc analysis. the recovery (r) of microdialysis sampling was measured in vitro under similar conditions as 65.74% for cu(ii), 70.45% for zn(ii) with r.s.d. below 3.2%. the primary binding constants and number of binding site estimated by the scatchard plot analysis are 5.04 x 10(6) m-1 and 0.85 for cu(ii), and 9.87 x 10(6) m-1 and 1.10 for zn(ii), respectively. the competition of cu(ii) and zn(ii) at the second binding site on hsa was investigated, and it was observed that there is a second site on hsa to bind cu(ii) and zn(ii), the affinity of cu(ii) is stronger than that of zn(ii) to this second site of hsa. (c) 2001 elsevier science b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | serum-albumin ; site ; equilibrium ; copper(ii) ; constants ; complex |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000170860300010 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/83721]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, M,Zou, HF,Wang, HL,et al. Binding of metal ions with protein studied by a combined technique of microdialysis with liquid chromatography[J]. analytica chimica acta,2001,443(1):91-99. |
| APA | Guo, M,Zou, HF,Wang, HL,Kong, L,&Ni, JY.(2001).Binding of metal ions with protein studied by a combined technique of microdialysis with liquid chromatography.analytica chimica acta,443(1),91-99. |
| MLA | Guo, M,et al."Binding of metal ions with protein studied by a combined technique of microdialysis with liquid chromatography".analytica chimica acta 443.1(2001):91-99. |
入库方式： OAI收割
来源：大连化学物理研究所
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