Direct determination of sialic acids in serum by capillary electrophoresis with UV detection
文献类型：期刊论文
| 作者 | Dong, XL; Xu, Y; Lin, BC |
| 刊名 | chromatographia
 |
| 出版日期 | 2000-07-01 |
| 卷号 | 52期号:1-2页码:14-16 |
| 关键词 | capillary electrophoresis sialic acids human serum |
| 英文摘要 | a novel method for the determination of n-acetylneuraminic acid (nana) and n-glycolylneuraminic acid (ngna) has been developed using high-performance capillary electrophoresis with uv detection at 195 nm, without pre or post-column derivatisation. the acids were separated in a 50-cm, fused-silica capillary (50 mu m i.d, 45.5-cm effective length) with na2b4o7-na2hpo4 buffer. the detection limit for nana is a concentration of 9.6 x 10(-6) m or, in terms of mass: 3.879 x 10(-14) mol (39 fmol). this method is applicable to determination of nana in normal human serum. the results were also compared with those of the colorimetrie method. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; n-glycolylneuraminic acids ; lung-cancer ; assay |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000088514900002 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/84005]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Dalian Third Municipal Hosp, Dalian 116033, Peoples R China 3.Dalian Obstet & Gynecol Hosp, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Dong, XL,Xu, Y,Lin, BC. Direct determination of sialic acids in serum by capillary electrophoresis with UV detection[J]. chromatographia,2000,52(1-2):14-16. |
| APA | Dong, XL,Xu, Y,&Lin, BC.(2000).Direct determination of sialic acids in serum by capillary electrophoresis with UV detection.chromatographia,52(1-2),14-16. |
| MLA | Dong, XL,et al."Direct determination of sialic acids in serum by capillary electrophoresis with UV detection".chromatographia 52.1-2(2000):14-16. |
入库方式： OAI收割
来源：大连化学物理研究所
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