Study of normal and modified nucleosides in serum by RP-HPLC
文献类型：期刊论文
| 作者 | Xu, G; Enderle, H; Liebich, H; Lu, P |
| 刊名 | chromatographia
 |
| 出版日期 | 2000-08-01 |
| 卷号 | 52期号:3-4页码:152-158 |
| 关键词 | column liquid chromatography nucleosides in serum tumor markers factor analysis |
| 英文摘要 | modified nucleosides derived predominantly from transfer ribonucleic acid (trna) have been studied as possible tumor markers. in this paper a reversed-phase high-performance liquid chromatographic (rp-hplc) method has been applied to study 15 normal and modified nucleosides in serum. the nucleoside levels in normal human serum were established, and the concentrations of 15 nucleosides in serum from 19 cancer patients were determined, it was found that the concentrations of modified nucleosides were significantly higher in patient sera. based on 15 nucleoside concentrations in serum, factor analysis could classify correctly 90% of cancer patients from the normal humans further work showed that urine was slightly better than serum when determining nucleosides as biological marker candidates. more work is ongoing to determine the clinical usefulness of modified nucleosides as tumor markers. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; placental alkaline-phosphatase ; urinary modified nucleosides ; cancer-patients ; tumor-markers ; transfer-rna ; capillary-electrophoresis ; carcinoembryonic antigen ; monoclonal-antibodies ; pseudouridine |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000088867200005 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/84015]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Univ Tubingen, Med Klin, D-72076 Tubingen, Germany |
| 推荐引用方式 GB/T 7714 | Xu, G,Enderle, H,Liebich, H,et al. Study of normal and modified nucleosides in serum by RP-HPLC[J]. chromatographia,2000,52(3-4):152-158. |
| APA | Xu, G,Enderle, H,Liebich, H,&Lu, P.(2000).Study of normal and modified nucleosides in serum by RP-HPLC.chromatographia,52(3-4),152-158. |
| MLA | Xu, G,et al."Study of normal and modified nucleosides in serum by RP-HPLC".chromatographia 52.3-4(2000):152-158. |
入库方式： OAI收割
来源：大连化学物理研究所
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