Capillary electrophoretic study on UDP-sugars in cells
文献类型：期刊论文
| 作者 | Xu, GW; Lehmann, R; Lu, X; Liebich, HM; Zhang, YK; Lu, PZ |
| 刊名 | science in china series b-chemistry
 |
| 出版日期 | 2000-02-01 |
| 卷号 | 43期号:1页码:85-93 |
| 关键词 | cells glucose capillary electrophoresis UDP-sugars hexosamines |
| 英文摘要 | glucose is an important regulator of cell growth and metabolism. uridine diphosphate sugars (udp-sugars), as the intermediate products of metabolism, play pivotal roles as precursors in the synthesis of complex carbohydrates and glycolipids as well as lectose. it is very important to study their metabolism in cells in clinical biochemistry. a capillary electrophoretic method has been developed for the analysis of udp-sugars and nucleotides, by using an uncoated capillary (70cm x 50 mu m) and 20 mmol/l borax buffer (ph 9), 4 important udp-sugars can be analyzed in 15 min at 22 kv with satisfactory precision and sensitivity. the developed method has been applied to analyze udp-sugars concentrations in lymphocytes, fibroblasts and mesangial cells, and the results show it not only is much better than hplc method, but also can be used to measure the energy charge of cells. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | performance liquid-chromatography ; blood-cells ; granulocytes ; udpgalactose ; nucleotides ; separation ; metabolism ; udpglucose ; growth |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000085514600011 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/84017]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Lab Chromatog, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China 2.Univ Tubingen, Med Klin, D-72076 Tubingen, Germany |
| 推荐引用方式 GB/T 7714 | Xu, GW,Lehmann, R,Lu, X,et al. Capillary electrophoretic study on UDP-sugars in cells[J]. science in china series b-chemistry,2000,43(1):85-93. |
| APA | Xu, GW,Lehmann, R,Lu, X,Liebich, HM,Zhang, YK,&Lu, PZ.(2000).Capillary electrophoretic study on UDP-sugars in cells.science in china series b-chemistry,43(1),85-93. |
| MLA | Xu, GW,et al."Capillary electrophoretic study on UDP-sugars in cells".science in china series b-chemistry 43.1(2000):85-93. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。