Capillary electrophoresis of urinary normal and modified nucleosides of cancer patients
文献类型：期刊论文
| 作者 | Liebich, HM; Xu, G; Di Stefano, C; Lehmann, R |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 1998-01-16 |
| 卷号 | 793期号:2页码:341-347 |
| 关键词 | nucleosides |
| 英文摘要 | this paper gives a capillary electrophoretic method for the separation of 15 urinary normal and modified nucleosides from cancer patients in less than 40 min. a 500 mmx50 mu m uncoated capillary column (437.5 mm to window) was used. the effects of the voltage and the sodium dodecyl sulfate (sds) concentration in the buffer on the separation were studied. with reproducibilities of migration times better than 1.2% (r.s.d.) and determined concentrations better than 5-25%, depending on the concentrations of nucleosides in the urine, the analytical characteristics of the method were food. using this developed method, the concentrations of 13 normal and modified nucleosides, extracted on a phenyl boronic acid affinity chromatography column, in 25 urines from patients of 14 kinds of cancer were determined. the levels (nmol/mol creatinine) of modified nucleosides in urines from cancer patients were increased as compared with those in normal urines. (c) 1998 elsevier science b.v. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | placental alkaline-phosphatase ; tissue polypeptide antigen ; small cell-carcinoma ; tumor-markers ; carcinoembryonic antigen ; transfer-rna ; monoclonal-antibodies ; biological markers ; ribonucleic-acid ; excretion |
| 收录类别 | ISTP ; SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000071668800013 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/84045]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Univ Tubingen, Med Klin, Abt 4, Dept Clin Chem, D-72076 Tubingen, Germany 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liebich, HM,Xu, G,Di Stefano, C,et al. Capillary electrophoresis of urinary normal and modified nucleosides of cancer patients[J]. journal of chromatography a,1998,793(2):341-347. |
| APA | Liebich, HM,Xu, G,Di Stefano, C,&Lehmann, R.(1998).Capillary electrophoresis of urinary normal and modified nucleosides of cancer patients.journal of chromatography a,793(2),341-347. |
| MLA | Liebich, HM,et al."Capillary electrophoresis of urinary normal and modified nucleosides of cancer patients".journal of chromatography a 793.2(1998):341-347. |
入库方式： OAI收割
来源：大连化学物理研究所
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