Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis
文献类型：期刊论文
| 作者 | He, XY; Xiao, HB; Liang, XM; Lin, BC |
| 刊名 | journal of separation science
 |
| 出版日期 | 2002-07-01 |
| 卷号 | 25期号:10-11页码:711-714 |
| 关键词 | capillary zone electrophoresis interaction pUC19DNA ovalbumin binding constant |
| 英文摘要 | capillary zone electrophoresis (cze) was used to study the interaction between puc19dna (puc19) and ovalbumin (ova). samples containing puc19 and ova at various ratios were incubated at room temperature and were then separated by cze with tris-acetate buffer at ph 7.2. reduction in ultraviolet (uv) absorbance of puc19 was due to the decrease of free puc19 after binding to ova. the binding constant of the interaction calculated from the scatchard plot was (1.46+/-0.15) x 10(5) m-1. the use of polyacrylamide-coated capillary showed better effects than that of uncoated capillary. the results show that it is important to keep a constant ionic strength in the samples in order to obtain accurate quantitative data in binding assays by cze. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | binding assays ; dna-binding ; protein ; tool |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000177594400012 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/84185]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, XY,Xiao, HB,Liang, XM,et al. Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis[J]. journal of separation science,2002,25(10-11):711-714. |
| APA | He, XY,Xiao, HB,Liang, XM,&Lin, BC.(2002).Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis.journal of separation science,25(10-11),711-714. |
| MLA | He, XY,et al."Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis".journal of separation science 25.10-11(2002):711-714. |
入库方式： OAI收割
来源：大连化学物理研究所
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