中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis

文献类型:期刊论文

作者He, XY; Xiao, HB; Liang, XM; Lin, BC
刊名journal of separation science
出版日期2002-07-01
卷号25期号:10-11页码:711-714
关键词capillary zone electrophoresis interaction pUC19DNA ovalbumin binding constant
英文摘要capillary zone electrophoresis (cze) was used to study the interaction between puc19dna (puc19) and ovalbumin (ova). samples containing puc19 and ova at various ratios were incubated at room temperature and were then separated by cze with tris-acetate buffer at ph 7.2. reduction in ultraviolet (uv) absorbance of puc19 was due to the decrease of free puc19 after binding to ova. the binding constant of the interaction calculated from the scatchard plot was (1.46+/-0.15) x 10(5) m-1. the use of polyacrylamide-coated capillary showed better effects than that of uncoated capillary. the results show that it is important to keep a constant ionic strength in the samples in order to obtain accurate quantitative data in binding assays by cze.
WOS标题词science & technology ; physical sciences
类目[WOS]chemistry, analytical
研究领域[WOS]chemistry
关键词[WOS]binding assays ; dna-binding ; protein ; tool
收录类别SCI
原文出处true
语种英语
WOS记录号WOS:000177594400012
公开日期2010-11-30
源URL[http://159.226.238.44/handle/321008/84185]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China
推荐引用方式
GB/T 7714
He, XY,Xiao, HB,Liang, XM,et al. Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis[J]. journal of separation science,2002,25(10-11):711-714.
APA He, XY,Xiao, HB,Liang, XM,&Lin, BC.(2002).Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis.journal of separation science,25(10-11),711-714.
MLA He, XY,et al."Quantitative evaluation of the interaction between pUC19DNA and ovalbumin by capillary zone electrophoresis".journal of separation science 25.10-11(2002):711-714.

入库方式: OAI收割

来源:大连化学物理研究所

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