Interaction between netropsin and double-stranded DNA in capillary zone electrophoresis and affinity capillary electrophoresis
文献类型：期刊论文
| 作者 | He, XY; Li, DZ; Liang, AY; Lin, BC |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2002-12-27 |
| 卷号 | 982期号:2页码:285-291 |
| 关键词 | affinity capillary electrophoresis DNA netropsin |
| 英文摘要 | capillary zone electrophoresis (cze) and affinity capillary electrophoresis (ace) were applied to study the interaction between netropsin and a 14mer double-stranded dna (dsdna). the use of a polyacrylamide coated capillary can suppress the electroosmotic flow (eof) and the adsorption of dna onto the wall. better analysis of the dna was achieved in a coated capillary upon tris-acetate. in cze, the peak width broadened due to the affinity interaction between dsdna and netropsin. in ace, o-toluic acid, a negatively charged molecule was used as the indicator to monitor the changes of eof when netropsin was added to the running buffer. the 14mer dsdna showed different mobilities upon various concentrations of netropsin due to the affinity interaction between the dsdna and netropsin. the binding constants of this interaction were (1.07 +/- 0.10) . 10(5) m-1 calculated from cze and (4.75 +/- 0.30) . 10(4) m-1 from ace using a scatchard plot. the binding stoichiometry was 1:1 calculated from cze which was superior to ace in this study. (c) 2002 elsevier science b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | raman-spectroscopy ; binding ligands ; minor-groove ; recognition ; acid ; tool |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000179485200011 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/84203]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, XY,Li, DZ,Liang, AY,et al. Interaction between netropsin and double-stranded DNA in capillary zone electrophoresis and affinity capillary electrophoresis[J]. journal of chromatography a,2002,982(2):285-291. |
| APA | He, XY,Li, DZ,Liang, AY,&Lin, BC.(2002).Interaction between netropsin and double-stranded DNA in capillary zone electrophoresis and affinity capillary electrophoresis.journal of chromatography a,982(2),285-291. |
| MLA | He, XY,et al."Interaction between netropsin and double-stranded DNA in capillary zone electrophoresis and affinity capillary electrophoresis".journal of chromatography a 982.2(2002):285-291. |
入库方式： OAI收割
来源：大连化学物理研究所
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