Competing binding of metal ions with protein studied by microdialysis
文献类型：期刊论文
| 作者 | Guo, M; Kong, L; Mao, XQ; Li, X; Zou, HF |
| 刊名 | science in china series b-chemistry
 |
| 出版日期 | 2002-04-01 |
| 卷号 | 45期号:2页码:151-157 |
| 关键词 | microdialysis high performance liquid chromatography human serum albumin metal ions competing binding |
| 英文摘要 | a method has been established to study the competing binding of metal ions with protein by a combined technique of microdialysis with high performance liquid chromatography (hplc). ni2+, cd2+, zn2+, cu2+ and human serum albumin (hsa) were chosen as model metal ions and protein. the experimental results show that ni2+ and cu2+ share a common primary binding site on hsa, and zn2+ and cd2+ share a different common primary binding site from them, but there is a common multi-metal binding site for all of those four metal ions. this method show advantages of fast sampling, easily to be operated and especially to be useful when ideal spectroscopic probes are not available for the study of interaction between protein and metal ions. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | human-serum-albumin ; performance liquid-chromatography ; site |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000175009000006 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/84213]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Acad Sinica, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116011, Peoples R China 2.Dalian Univ, Dept Chem & Chem Technol, Dalian 116622, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, M,Kong, L,Mao, XQ,et al. Competing binding of metal ions with protein studied by microdialysis[J]. science in china series b-chemistry,2002,45(2):151-157. |
| APA | Guo, M,Kong, L,Mao, XQ,Li, X,&Zou, HF.(2002).Competing binding of metal ions with protein studied by microdialysis.science in china series b-chemistry,45(2),151-157. |
| MLA | Guo, M,et al."Competing binding of metal ions with protein studied by microdialysis".science in china series b-chemistry 45.2(2002):151-157. |
入库方式： OAI收割
来源：大连化学物理研究所
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