细菌二氯甲烷脱氯酶基因对拟南芥的转化
文献类型：期刊论文
| 作者 | 郝林,曹军,冯树 |
| 刊名 | 应用与环境生物学报
 |
| 出版日期 | 2001-02-28 |
| 期号 | 01页码:76-78 |
| 关键词 | 二氯甲烷脱氯酶基因 拟南芥 转化(图4参9) |
| 中文摘要 | The dichloromethane dehalogenase gene (dcm) of Methylophilus was cloned into the plasmid pKYLX71-35S2 and fused with the 35S2 promotor,then transformed Arabidopsis by the infiltration mediated with Agrobacterium tumefaciens. Homozygous dcm seeds were obtained after several generations selection on the medium with 50 mg L -1 kanamycin.Northern blotting showed dcm mRNA was high in cytoplasm and the detection of DCM enzyme indicated that the lines containing high dcm mRNA expressed high DCM enzyme activities. Fig 4, Ref 9 |
| 收录类别 | CNKI |
| 语种 | 中文 |
| 公开日期 | 2011-05-05 ; 2011-05-20 |
| 源URL | [http://210.72.129.5/handle/321005/52896]  |
| 专题 | 沈阳应用生态研究所_沈阳应用生态研究所_期刊论文 |
| 推荐引用方式 GB/T 7714 | 郝林,曹军,冯树. 细菌二氯甲烷脱氯酶基因对拟南芥的转化[J]. 应用与环境生物学报,2001(01):76-78. |
| APA | 郝林,曹军,冯树.(2001).细菌二氯甲烷脱氯酶基因对拟南芥的转化.应用与环境生物学报(01),76-78. |
| MLA | 郝林,曹军,冯树."细菌二氯甲烷脱氯酶基因对拟南芥的转化".应用与环境生物学报 .01(2001):76-78. |
入库方式： OAI收割
来源：沈阳应用生态研究所
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