Generation of beta-lactoglobulin-modified transgenic goats by homologous recombination
文献类型：期刊论文
| 作者 | Zhu, Hongmei1,2; Hu, Linyong1,3; Liu, Jun1,2; Chen, Huatao1,2; Cui, Chenchen1,2; Song, Yujie1,2; Jin, Yaping1,2; Zhang, Yong1,2 |
| 刊名 | febs journal
 |
| 出版日期 | 2016-12-01 |
| 卷号 | 283期号:24页码:4600-4613 |
| 关键词 | homologous recombination milk allergy somatic cell nuclear transfer transgenic goat beta-lactoglobulin |
| 英文摘要 | beta-lactoglobulin (blg) is a dominant allergen present in the milk of goats and other ungulates, although it is not found in human breast milk. thus, the presence of blg restricts the consumption of goat's milk by humans. in the present study, we examined whether the disruption of the blg gene in goats by homologous recombination (hr) reduced blg content in goat's milk and decreased the allergic response to milk. in one approach, exon 2 of the blg gene was efficiently targeted using hr with a blg knockout vector. in a second approach to disrupt blg gene expression and drive exogenous human alpha-lactalbumin (hla) gene expression, two hla knock-in constructs were used to target exons 1-4 of the blg gene via hr, and expression of hla was then confirmed in goat mammary epithelial cells in vitro. the recombinant clones from both approaches were then used for somatic cell nuclear transfer, generating two transgenic goats possessing a blg knockout allele or site-specific hla integration allele. milk assays demonstrated a reduction in blg levels in both the blg knockout and hla knock-in goats; furthermore, hla was present in the hla knock-in goat's milk. allergenic analysis in mice indicated that the transgenic goat's milk was less allergenic than wild-type goat's milk. these results support the development of gene-targeted animals as an effective tool for reducing allergic reactions to milk and improving nutrition. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | mammary epithelial-cells ; alpha-lactalbumin ; nuclear transfer ; mammalian-cells ; gene-expression ; cows milk ; allergy ; allergenicity ; lactation ; increases |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000392744800015 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6780]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China 2.Northwest A&F Univ, Minist Agr, Key Lab Anim Biotechnol, Yangling, Shaanxi, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Hongmei,Hu, Linyong,Liu, Jun,et al. Generation of beta-lactoglobulin-modified transgenic goats by homologous recombination[J]. febs journal,2016,283(24):4600-4613. |
| APA | Zhu, Hongmei.,Hu, Linyong.,Liu, Jun.,Chen, Huatao.,Cui, Chenchen.,...&Zhang, Yong.(2016).Generation of beta-lactoglobulin-modified transgenic goats by homologous recombination.febs journal,283(24),4600-4613. |
| MLA | Zhu, Hongmei,et al."Generation of beta-lactoglobulin-modified transgenic goats by homologous recombination".febs journal 283.24(2016):4600-4613. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。