Two-dimensional chromatography based on on-line HPLC-DPPH bioactivity-guided assay for the preparative isolation of analogue antioxidant compound from Arenaria kansuensis
文献类型：期刊论文
| 作者 | Cui, Yulei1,2,3,4; Shen, Na1,2,3,4; Yuan, Xiang1,2,3,4; Dang, Jun1,2,3; Shao, Yun1,2,3; Mei, Lijuan1,2,3; Tao, Yanduo1,2,3; Wang, Qilan1,2,3; Liu, Zenggen1,2,3 |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2017-03-01 |
| 卷号 | 1046页码:81-86 |
| 关键词 | Two-dimensional separation Arenaria kansuensis HPLC-DPPH-DAD Antioxidant |
| 英文摘要 | traditional tibetan medicine is important for discovery of drug precursors. however, information about the chemical composition of traditional tibetan medicine is very limited due to the lack of appropriate chromatographic purification methods. in the present work, a. kansuensis was taken as an example and a novel two-dimensional reversed-phase/hydrophilic interaction liquid chromatography(hilic) method based on on-line hplc-dpph bioactivity-guided assay was developed for the purification of analogue antioxidant compounds with high purity from the extract of a. kansuensis. based on the separation results of many different chromatographic stationary phases, the first-dimensional (1d) preparation was carried on a rp-c18hce prep column, and 2 antioxidant fractions were obtained from the 800 mg crude sample with a recovery of 56.7%. a hilic-xamide prep column was selected for the second-dimensional (2d) preparation. finally, a novel antioxidant beta-carboline alkaloids (glusodichotomine ak) and 4 known compounds (tricin, homoeriodictyol, luteolin, glucodichotomine b) were purified from a. kansuensis. the purity of the compounds isolated from the crude extract was >98%, which indicated that the method built in this work was efficient to manufacture single analogue antioxidant compounds of high purity from the extract of a. kansuensis. additionally, this method showed great potential in the preparation of analogue structure antioxidant compounds and can serve as a good example for the purification of analogue structure antioxidant carboline alkaloids and flavonoids from other plant materials. (c) 2017 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | beta-carboline alkaloids ; liquid-chromatography ; anthocyanins ; constituents ; diseases |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000395602100010 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6795]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining 810008, Qinghai, Peoples R China 2.Chinese Acad Sci, Key Lab Tibetan Med Res, Xining 810008, Qinghai, Peoples R China 3.Key Lab Tibetan Med Res Qinghai Prov, Xining 810008, Qinghai, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cui, Yulei,Shen, Na,Yuan, Xiang,et al. Two-dimensional chromatography based on on-line HPLC-DPPH bioactivity-guided assay for the preparative isolation of analogue antioxidant compound from Arenaria kansuensis[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2017,1046:81-86. |
| APA | Cui, Yulei.,Shen, Na.,Yuan, Xiang.,Dang, Jun.,Shao, Yun.,...&Liu, Zenggen.(2017).Two-dimensional chromatography based on on-line HPLC-DPPH bioactivity-guided assay for the preparative isolation of analogue antioxidant compound from Arenaria kansuensis.journal of chromatography b-analytical technologies in the biomedical and life sciences,1046,81-86. |
| MLA | Cui, Yulei,et al."Two-dimensional chromatography based on on-line HPLC-DPPH bioactivity-guided assay for the preparative isolation of analogue antioxidant compound from Arenaria kansuensis".journal of chromatography b-analytical technologies in the biomedical and life sciences 1046(2017):81-86. |
入库方式： OAI收割
来源：西北高原生物研究所
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