Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS
文献类型：期刊论文
| 作者 | Chen, Tao1,2; Li, Hongmei1,2,3; Tan, Liang1,2; Li, Yulin1,2 |
| 刊名 | separation science and technology
 |
| 出版日期 | 2017 |
| 卷号 | 52期号:8页码:1423-1429 |
| 关键词 | 4 '-Demethyldeoxypodophyllotoxin high-speed counter-current chromatography HPLC-MS minor lignan Sinopodophyllum emodi |
| 英文摘要 | a minor lignan, 4'-demethyldeoxypodophyllotoxin, was isolated from sinopodophyllum emodi. first, a crude extract of s. emodi was analyzed by high performance liquid chromatography-mass spectrometry (hplc-ms) to confirm the presence of the target compound. then, the target lignan was successfully enriched in the crude extract precipitate by medium-pressure lc (mplc) with high recovery (85%). finally, an efficient separation was achieved by high-speed counter-current chromatography (hsccc) using a two-phase solvent system of n-hexane-ethyl acetate-methanol-water (1:1:1:1, v/v/v/v). these results clearly demonstrate that the combination of hplc-ms, mplc, and hsccc could be a powerful technique for the rapid screening, enrichment, and separation of minor compounds from complex natural products. |
| WOS标题词 | science & technology ; physical sciences ; technology |
| 类目[WOS] | chemistry, multidisciplinary ; engineering, chemical |
| 研究领域[WOS] | chemistry ; engineering |
| 关键词[WOS] | performance liquid-chromatography ; macroporous resin ; mass-spectrometry ; rheum-tanguticum ; rapid enrichment ; natural-products ; purification ; extraction ; glycosides ; lignans |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000399716200011 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6815]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, Northwest Plateau Inst Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining, Peoples R China 2.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining, Peoples R China 3.Univ Chinese Acad Sci, Savaid Med Sch, Beijing, P. R. China |
| 推荐引用方式 GB/T 7714 | Chen, Tao,Li, Hongmei,Tan, Liang,et al. Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS[J]. separation science and technology,2017,52(8):1423-1429. |
| APA | Chen, Tao,Li, Hongmei,Tan, Liang,&Li, Yulin.(2017).Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS.separation science and technology,52(8),1423-1429. |
| MLA | Chen, Tao,et al."Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS".separation science and technology 52.8(2017):1423-1429. |
入库方式： OAI收割
来源：西北高原生物研究所
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