中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS

文献类型:期刊论文

作者Chen, Tao1,2; Li, Hongmei1,2,3; Tan, Liang1,2; Li, Yulin1,2
刊名separation science and technology
出版日期2017
卷号52期号:8页码:1423-1429
关键词4 '-Demethyldeoxypodophyllotoxin high-speed counter-current chromatography HPLC-MS minor lignan Sinopodophyllum emodi
英文摘要a minor lignan, 4'-demethyldeoxypodophyllotoxin, was isolated from sinopodophyllum emodi. first, a crude extract of s. emodi was analyzed by high performance liquid chromatography-mass spectrometry (hplc-ms) to confirm the presence of the target compound. then, the target lignan was successfully enriched in the crude extract precipitate by medium-pressure lc (mplc) with high recovery (85%). finally, an efficient separation was achieved by high-speed counter-current chromatography (hsccc) using a two-phase solvent system of n-hexane-ethyl acetate-methanol-water (1:1:1:1, v/v/v/v). these results clearly demonstrate that the combination of hplc-ms, mplc, and hsccc could be a powerful technique for the rapid screening, enrichment, and separation of minor compounds from complex natural products.
WOS标题词science & technology ; physical sciences ; technology
类目[WOS]chemistry, multidisciplinary ; engineering, chemical
研究领域[WOS]chemistry ; engineering
关键词[WOS]performance liquid-chromatography ; macroporous resin ; mass-spectrometry ; rheum-tanguticum ; rapid enrichment ; natural-products ; purification ; extraction ; glycosides ; lignans
收录类别SCI
语种英语
WOS记录号WOS:000399716200011
源URL[http://ir.nwipb.ac.cn/handle/363003/6815]  
专题西北高原生物研究所_中国科学院西北高原生物研究所
作者单位1.Chinese Acad Sci, Northwest Plateau Inst Biol, Qinghai Key Lab Qinghai Tibet Plateau Biol Resour, Xining, Peoples R China
2.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Tibetan Med Res, Xining, Peoples R China
3.Univ Chinese Acad Sci, Savaid Med Sch, Beijing, P. R. China
推荐引用方式
GB/T 7714
Chen, Tao,Li, Hongmei,Tan, Liang,et al. Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS[J]. separation science and technology,2017,52(8):1423-1429.
APA Chen, Tao,Li, Hongmei,Tan, Liang,&Li, Yulin.(2017).Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS.separation science and technology,52(8),1423-1429.
MLA Chen, Tao,et al."Separation of 4 '-demethyldeoxypodophyllotoxin from Sinopodophyllum emodi by medium-pressure LC and high-speed counter-current chromatography guided by HPLC-MS".separation science and technology 52.8(2017):1423-1429.

入库方式: OAI收割

来源:西北高原生物研究所

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