Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Fang, Zhijia1; Chen, Zhongxiang1; Wang, Song2; Shi, Ping2; Shen, Yuhu3; Zhang, Youshang1; Xiao, Junhua1; Huang, Zhiwei1 |
| 刊名 | applied and environmental microbiology
 |
| 出版日期 | 2017 |
| 卷号 | 83期号:1 |
| 关键词 | cadmium OLE1 delta-9 desaturase unsaturated fatty acids cytoplasmic membrane |
| 英文摘要 | the heavy metal cadmium is widely used and released into the environment, posing a severe threat to crops and humans. saccharomyces cerevisiae is one of the most commonly used organisms in the investigation of environmental metal toxicity. we investigated cadmium stress and the adaptive mechanisms of yeast by screening a genome-wide essential gene overexpression library. a candidate gene, ole1, encodes a delta-9 desaturase and was associated with high anti-cadmium-stress activity. the results demonstrated that the expression of ole1 was positively correlated with cadmium stress tolerance and induction was independent of mga2p and spt23p (important regulatory factors for ole1). moreover, in response to cadmium stress, cellular levels of monounsaturated fatty acids were increased. the addition of exogenous unsaturated fatty acids simulated overexpression of ole1, leading to cadmium resistance. such regulation of ole1 in the synthesis of unsaturated fatty acids may serve as a positive feedback mechanism to help cells counter the lipid peroxidation and cytoplasmic membrane damage caused by cadmium. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | biotechnology & applied microbiology ; microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology ; microbiology |
| 关键词[WOS] | stearoyl-coa desaturase ; unsaturated fatty-acid ; lipid-composition ; plasma-membrane ; gene-expression ; cell-death ; yeast ; tolerance ; fluidity ; stress |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000393205400006 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6833]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Donghua Univ, Coll Chem Chem Engn & Biotechnol, Shanghai, Peoples R China 2.East China Univ Sci & Technol, Key Lab Bioreactor Engn, Shanghai, Peoples R China 3.Chinese Acad Sci, Northwest Inst Plateau Biol, Xining, Qinghai Provinc, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fang, Zhijia,Chen, Zhongxiang,Wang, Song,et al. Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae[J]. applied and environmental microbiology,2017,83(1). |
| APA | Fang, Zhijia.,Chen, Zhongxiang.,Wang, Song.,Shi, Ping.,Shen, Yuhu.,...&Huang, Zhiwei.(2017).Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae.applied and environmental microbiology,83(1). |
| MLA | Fang, Zhijia,et al."Overexpression of OLE1 Enhances Cytoplasmic Membrane Stability and Confers Resistance to Cadmium in Saccharomyces cerevisiae".applied and environmental microbiology 83.1(2017). |
入库方式： OAI收割
来源：西北高原生物研究所
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