Determination of free fatty acids by high performance liquid chromatography with fluorescence detection and. identification with mass spectrometry
文献类型：期刊论文
| 作者 | Zhu Fang; Zhao Wen-Chen; Zhao Xian-En; Suo You-Rui; Liu Su-Juan; You Jin-Mao |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2007-04-01 |
| 卷号 | 35期号:4页码:489-494 |
| 关键词 | high performance liquid chromatography/mass spectrometry fluorescence detection pre-column derivatization fatty acids rape bee pollen |
| 英文摘要 | on a reversed-phase eclipse xdb-c-8 chromatographic column, 20 fatty acids were derivatized using 2-(2-(anthracen-10-yl)-1h-naphtho[2,3-d] imidazol-1-yl) ethyl-p-toluenesulfonate (anits) as precolumn derivatization reagent and separated by hplc in conjunction with a gradient elution with fluorescence detection and ms identification. the reagent reacted with fatty acids in the presence of k2co3 catalyst at 90 degrees c for 40 min in n, n-dimethylformamide solvent to give maximum and constant fluorescence intensity with the addition of 7-fold molar reagent excess to total molar fatty acids. the fluorescence excitation and emission wavelengths of derivatives were lambda(ex) =250 mn and lambda(em) =512 nm, respectively. the identification of fatty acid derivatives was performed by the online apci/ms (atmospheric pressure chemical ionization source) in positive mode. all linear correlation coefficients for fatty acid derivatives are over 0. 9999, and detection limits, at signal-to-noise of 3:1, are 24. 76 - 98. 79 fmol for labeled fatty acids. the established method is sensitive and repeatable. it has been applied to for the determination of free fatty acids from rape bee pollen samples with satisfactory results. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | labeling reagent |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000246717800005 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6864]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu Fang,Zhao Wen-Chen,Zhao Xian-En,et al. Determination of free fatty acids by high performance liquid chromatography with fluorescence detection and. identification with mass spectrometry[J]. chinese journal of analytical chemistry,2007,35(4):489-494. |
| APA | Zhu Fang,Zhao Wen-Chen,Zhao Xian-En,Suo You-Rui,Liu Su-Juan,&You Jin-Mao.(2007).Determination of free fatty acids by high performance liquid chromatography with fluorescence detection and. identification with mass spectrometry.chinese journal of analytical chemistry,35(4),489-494. |
| MLA | Zhu Fang,et al."Determination of free fatty acids by high performance liquid chromatography with fluorescence detection and. identification with mass spectrometry".chinese journal of analytical chemistry 35.4(2007):489-494. |
入库方式： OAI收割
来源：西北高原生物研究所
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