Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization
文献类型：期刊论文
| 作者 | Ding Yang-Jun; Zhao Xian-En; Zhu Fang; Suo You-Rui; Li Yu-Lin; Chen Cui-Chen; You Jin-Mao |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2007-03-01 |
| 卷号 | 35期号:3页码:375-381 |
| 关键词 | high performance liquid chromatography/mass spectrometry pre-column derivatization fish oil fatty acids position of double bond |
| 英文摘要 | a method for the identification of long-chain unsaturated fatty acids in deep-sea fish oil using 1 2-benzo-3, 4-dihydrocarbazole-9-ethyl-benzenesulfonte (bdebs) as pre-column derivatization reagent by reversed-phase hplc/ms has been developed. to avoid the migration of double bond in the fatty acid chain, fish oil samples were saponificated and derivatized under nitrogen circumstance. the derivatized solution was extracted with hexane and dried under a stream of nitrogen; the residue was re-dissolved with acetorntrile prior to lc analysis. ms analysis of the fatty acid derivatives exhibited regular information regarding the position of the double bond in the fatty acid chain. with molecular foil mass at m/z [mh](+) and specific fragment ions obtained with ms analysis, the position of the double bond in fatty acid chain was located (or calculated) by means of several established formulas. according to the analysis of hplc/ms/apci, 23 fatty acids were identified. the main fatty acids in deep-sea fish oil are c-14-c-22 and 67.08% (area percent) of them are polytinsaturated fatly acids. the main components of the unsaturated fatty acids are c(16:1)9-hexadecenoic acid, 11.7% c16:4 4, 7, 10, 13-hexadecatetraenoic acid, 2.91%; c-18:1 12-octadecenoic acid, 11.1%; c-20:0 6, 9, 12, 15-octadecatetractioic acid, 3.62%; c-20:1 13-eicosenoic acid, 1.21%; c(20:5)5, 8, 11, 14, 17-eicosapentaenoic acid, 16.71%; c(22:6)2, 5, 8, 11, 14, 17-docosahexenoic acid, 10. 53%. the established method provides a new technical tool for the location of doulde bond positions in the, poly unsaturated fatty acids. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000245735200013 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6867]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding Yang-Jun,Zhao Xian-En,Zhu Fang,et al. Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization[J]. chinese journal of analytical chemistry,2007,35(3):375-381. |
| APA | Ding Yang-Jun.,Zhao Xian-En.,Zhu Fang.,Suo You-Rui.,Li Yu-Lin.,...&You Jin-Mao.(2007).Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization.chinese journal of analytical chemistry,35(3),375-381. |
| MLA | Ding Yang-Jun,et al."Identification of long-chain unsaturated fatty acids in deep-sea fish oil by liquid chromatography/mass spectrometry/atmospheric proessure chemical ionization".chinese journal of analytical chemistry 35.3(2007):375-381. |
入库方式： OAI收割
来源：西北高原生物研究所
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