Separation of derivatized carbohydrates by capillary zone electrophoresis
文献类型：期刊论文
| 作者 | Sheng Xiao1; Ding Chen-Xu2; Liu Ling-Jun1; Soo You-Rui2; Sun Zhi-Wei1; You Jin-Mao1,2 |
| 刊名 | chinese journal of analytical chemistry
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 36期号:3页码:280-284 |
| 关键词 | carbohydrates capillary zone electrophoresis 1-naphthyl-3-methyl-5-pyrazolone |
| 英文摘要 | a simple and rapid method was developed for the separation of derivatized carbohydrates using 1-naphthyl-3-methyl-5-pyrazolone (nmp) as derivatization reagent by capillary zone electrophoresis. the detection was performed with diode array at 254 nm. a 58.5 cm x 50 mu m i.d. ( 50 cm effective length) untreated fused-silica capillary was used. to optimize the conditions, the background electrolyte concentration, buffer ph, column temperature, voltage and other factors were studied. it was found that the buffer concentration and ph value have a greater impact on the separation, but the influences of temperature, voltage and additives on resolution were not obvious. on the basis of the optimization, the optimum conditions are as follows: 55 mmol/l borate buffer (ph 9.46), voltage 22 kv, column temperature at 20 degrees c and detection at 254 nm. the samples were atmospherically introduced with an injection time of 10 s. the results indicate that 9 nmp-derivatized carbohydrates achieve baseline resolution within 18 min under the proposed conditions. the tangufican nitratia sample was also analyzed under the optimum conditions. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254846900002 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6880]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Qufu Normal Univ, Coll Chem Sci, Qufu 273165, Peoples R China 2.Chinese Acad Sci, NW Plateau Inst Biol, Xining 810001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sheng Xiao,Ding Chen-Xu,Liu Ling-Jun,et al. Separation of derivatized carbohydrates by capillary zone electrophoresis[J]. chinese journal of analytical chemistry,2008,36(3):280-284. |
| APA | Sheng Xiao,Ding Chen-Xu,Liu Ling-Jun,Soo You-Rui,Sun Zhi-Wei,&You Jin-Mao.(2008).Separation of derivatized carbohydrates by capillary zone electrophoresis.chinese journal of analytical chemistry,36(3),280-284. |
| MLA | Sheng Xiao,et al."Separation of derivatized carbohydrates by capillary zone electrophoresis".chinese journal of analytical chemistry 36.3(2008):280-284. |
入库方式： OAI收割
来源：西北高原生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。