Genetic variation within and between populations of the Qinghai-Tibetan Plateau endemic Gentiana straminea (Gentianaceae) revealed by RAPD markers
文献类型：期刊论文
| 作者 | Li, Xiaojuan1; Yang, Huiling1,2; Liu, Jianquan1 |
| 刊名 | belgian journal of botany
 |
| 出版日期 | 2008 |
| 卷号 | 141期号:1页码:95-102 |
| 关键词 | Gentiana straminea genetic diversity population differentiation RAPD |
| 英文摘要 | gentiana straminea maxim. is an outcrossing perennial from the qinghai-tibetan plateau (qtp) that undergoes some selfing. this species has become threatened in recent years because of over-collection for medicinal use. a complete understanding of the partitioning of genetic variation between populations is needed to establish effective conservation measures for this species. in this study, we used rapds to quantify the genetic diversity of seven populations in the core distribution of g. straminea. of the 78 primers screened, 15 produced highly reproducible bands. using these primers, 127 discernible dna fragments were generated, of which 110 (86.61%) were polymorphic, indicating considerable genetic variation at the species level. however, there were relatively low levels of polymorphism at the population level, with the percentage of polymorphic bands and the average gene diversity ranging from 43.31% to 51.18% and from 0.175 to 0.200, respectively. the correlation between gene diversity and population size was only marginally significant. high genetic differentiation was found between populations (nei's genetic diversity analysis: g(st) = 0.408; amova analysis: phi(st) = 0.413). these differentiation measures were higher than that expected for an outbreeding species and the higher level of genetic partitioning between g. straminea populations may be a result of the combined effects of geitonogamous selfing, complex geography and past climatic oscillations. the high genetic differentiation detected between populations suggests that as many populations as possible should be included in any in situ or ex situ conservation program for this species. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 类目[WOS] | plant sciences |
| 研究领域[WOS] | plant sciences |
| 关键词[WOS] | south-american conifer ; seed production ; scabiosa-columbaria ; arbitrary primers ; central germany ; size ; diversity ; plant ; differentiation ; conservation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000259389100008 |
| 源URL | [http://ir.nwipb.ac.cn/handle/363003/6882]  |
| 专题 | 西北高原生物研究所_中国科学院西北高原生物研究所 |
| 作者单位 | 1.Chinese Acad Sci, NW 1nstitute Plateau Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810008, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiaojuan,Yang, Huiling,Liu, Jianquan. Genetic variation within and between populations of the Qinghai-Tibetan Plateau endemic Gentiana straminea (Gentianaceae) revealed by RAPD markers[J]. belgian journal of botany,2008,141(1):95-102. |
| APA | Li, Xiaojuan,Yang, Huiling,&Liu, Jianquan.(2008).Genetic variation within and between populations of the Qinghai-Tibetan Plateau endemic Gentiana straminea (Gentianaceae) revealed by RAPD markers.belgian journal of botany,141(1),95-102. |
| MLA | Li, Xiaojuan,et al."Genetic variation within and between populations of the Qinghai-Tibetan Plateau endemic Gentiana straminea (Gentianaceae) revealed by RAPD markers".belgian journal of botany 141.1(2008):95-102. |
入库方式： OAI收割
来源：西北高原生物研究所
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