Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow
文献类型：期刊论文
| 作者 | Yang H(杨浩); Li N(李宁) ; Du Y(杜宇); Tong CF(佟春芳); Lv SQ(吕守芹); Hu JR; Zhang Y(章燕); Long M(龙勉)
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| 刊名 | EXPERIMENTAL CELL RESEARCH
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| 出版日期 | 2017-02-01 |
| 卷号 | 351期号:1页码:91-99 |
| 关键词 | Neutrophils Liver sinusoids Shear flow Adhesive molecules Kupffer cells |
| ISSN号 | 0014-4827 |
| 中文摘要 | Neutrophil (polymorphonuclear leukocyte PMN) recruitment in the liver sinusoid takes place in almost all liver diseases and contributes to pathogen clearance or tissue damage. While PMN rolling unlikely appears in liver sinusoids and Mac-1 or CD44 is assumed to play respective roles during in vivo local or systematic inflammatory stimulation the regulating mechanisms of PMN adhesion and crawling dynamics are still unclear from those in vivo studies. Here we developed a two-dimensional in vitro sinusoidal model with primary liver sinusoidal endothelial cells (LSECs) and Kupffer cells (KCs) to investigate TNF-alpha-induced PMN recruitment under shear flow. Our data demonstrated that LFA-1 dominates the static or shear resistant adhesion of PMNs while Mac-1 decelerates PMN crawling on LSEC monolayer. Any one of LFA-1 Mac-1 and CD44 molecules is not able to work effectively for mediating PMN transmigration across LSEC monolayer. The presence of KCs only affects the randomness of PMN crawling. These findings further the understandings of PMN recruitment under shear flow in liver sinusoids. |
| 类目[WOS] | Oncology ; Cell Biology |
| 研究领域[WOS] | Oncology ; Cell Biology |
| 关键词[WOS] | BLOOD-BRAIN-BARRIER ; INFLAMED LIVER ; IN-VITRO ; HEPATIC MICROCIRCULATION ; MOLECULE-1 EXPRESSION ; LEUKOCYTE RECRUITMENT ; EXTRACELLULAR-MATRIX ; ISCHEMIA-REPERFUSION ; STERILE INFLAMMATION ; INJURY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000394196700011 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/60576]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Yang H,Li N,Du Y,et al. Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow[J]. EXPERIMENTAL CELL RESEARCH,2017,351(1):91-99. |
| APA | 杨浩.,李宁.,杜宇.,佟春芳.,吕守芹.,...&龙勉.(2017).Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow.EXPERIMENTAL CELL RESEARCH,351(1),91-99. |
| MLA | 杨浩,et al."Neutrophil adhesion and crawling dynamics on liver sinusoidal endothelial cells under shear flow".EXPERIMENTAL CELL RESEARCH 351.1(2017):91-99. |
入库方式： OAI收割
来源：力学研究所
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