Mechanical remodeling of normally sized mammalian cells under a gravity vector
文献类型：期刊论文
| 作者 | Zhang C ; Zhou LW(周吕文); Zhang F(张帆); Lv DY(吕东媛); Li N(李宁); Zheng L; Xu YH(徐艳红); Li Z; Sun SJ(孙树津); Long M(龙勉)
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| 刊名 | FASEB JOURNAL
 |
| 出版日期 | 2017-02-01 |
| 卷号 | 31期号:2页码:802-813 |
| 关键词 | gravity-directed mechanosensing nucleus translocation cytoskeletal remodeling FAC reorganization |
| ISSN号 | 0892-6638 |
| 中文摘要 | Translocation of the dense nucleus along a gravity vector initiates mechanical remodeling of a cell but the underlying mechanisms of cytoskeletal network and focal adhesion complex (FAC) reorganization in a mammalian cell remain unclear. We quantified the remodeling of an MC3T3-E1 cell placed in upward- downward- or edge-on-orientated substrate. Nucleus longitudinal translocation presents a high value in downward orientation at 24 h or in edge-on orientation at 72 h which is consistent with orientation-dependent distribution of perinuclear actin stress fibers and vimentin cords. Redistribution of total FAC area and fractionized super mature adhesion number coordinates this dependence at short duration. This orientation-dependent remodeling is associated with nucleus flattering and lamin A/C phosphorylation. Actin depolymerization or Rho-associated protein kinase signaling inhibition abolishes the orientation dependence of nucleus translocation whereas tubulin polymerization inhibition or vimentin disruption reserves the dependence. A biomechanical model is therefore proposed for integrating the mechanosensing of nucleus translocation with cytoskeletal remodeling and FAC reorganization induced by a gravity vector. |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biology ; Cell Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Life Sciences & Biomedicine - Other Topics ; Cell Biology |
| 关键词[WOS] | FOCAL ADHESION DYNAMICS ; ROS 17/2.8 CELLS ; NANOSCALE ARCHITECTURE ; ACTIN ; FORCE ; MECHANOTRANSDUCTION ; CYTOSKELETON ; STRESS ; TALIN ; MICROTUBULES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000394235400032 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/60581]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Zhang C,Zhou LW,Zhang F,et al. Mechanical remodeling of normally sized mammalian cells under a gravity vector[J]. FASEB JOURNAL,2017,31(2):802-813. |
| APA | Zhang C.,周吕文.,张帆.,吕东媛.,李宁.,...&龙勉.(2017).Mechanical remodeling of normally sized mammalian cells under a gravity vector.FASEB JOURNAL,31(2),802-813. |
| MLA | Zhang C,et al."Mechanical remodeling of normally sized mammalian cells under a gravity vector".FASEB JOURNAL 31.2(2017):802-813. |
入库方式： OAI收割
来源：力学研究所
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