Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation
文献类型：期刊论文
| 作者 | Li N(李宁) ; Mao DB(毛德斌); Gong YX(龚一心); Lv SQ(吕守芹); Zhang Y(章燕); Long M(龙勉)
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| 刊名 | Chinese Journal of Biomedical Engineering
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| 出版日期 | 2015 |
| 卷号 | 24期号:1页码:40-46 |
| 中文摘要 | LFA-1 and Mac-1, two β2integrin members constitutively expressed on neutrophils, mediate leukocyte recruitment cascade by binding to the same ligand of ICAM-1. The slow rolling and firm adhesion of leukocytes rely on LFA-1 while the cell crawling is dependent on Mac-1. We hypothesized that their distinct roles are likely attributed to the differences in the binding kinetics or in the diverse responses of outside-in and inside-out signaling. In this study, we compared the ICAM-1 binding features between soluble or membrane-expressed LFA-1 and Mac-1 with different affinity conformation using optical trap technique. Our data indicated that the affinity up-regulation from wide type(WT) to high affinity(HA) is off-rate dependent for LFA-1but on-rate dependent for Mac-1. The structural bases of this new finding were found to be consistent with our previous simulations. These results furthered our understanding in their function differences under shear flow. |
| 语种 | 英语 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/60641]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 推荐引用方式 GB/T 7714 | Li N,Mao DB,Gong YX,et al. Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation[J]. Chinese Journal of Biomedical Engineering,2015,24(1):40-46. |
| APA | Li N,Mao DB,Gong YX,Lv SQ,Zhang Y,&Long M.(2015).Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation.Chinese Journal of Biomedical Engineering,24(1),40-46. |
| MLA | Li N,et al."Distinct Kinetics Features of LFA-1 and Mac-1 in Neutrophil Activation".Chinese Journal of Biomedical Engineering 24.1(2015):40-46. |
入库方式： OAI收割
来源：力学研究所
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