Selectivity of biopolymer membranes using HepG2 cells
文献类型：期刊论文
| 作者 | Lv DY(吕东媛); Gao YX(高宇欣) ; Luo CH(罗春花); Lv SQ(吕守芹); Wang Q(汪迁); Xu XH(许向红); Sun SJ(孙树津); Wang CZ(王成之); Long M(龙勉)
|
| 刊名 | Regenerative Biomaterials
 |
| 出版日期 | 2015-02-10 |
| 卷号 | 2期号:1页码:21-29 |
| 关键词 | HepG2, biocompatible membrane bioartificial liver |
| 中文摘要 | Bioartificial liver (BAL) system has emerged as an alternative treatment to bridge acute liver failure to either liver transplantation or liver regeneration. One of the main reasons that the efficacy of the current BAL systems was not convincing in clinical trials is attributed to the lack of friendly interface between the membrane and the hepatocytes in liver bioreactor, the core unit of BAL system. Here, we systematically compared the biological responses of hepatosarcoma HepG2 cells seeded on eight, commercially available biocompatible membranes made of acetyl cellulose–nitrocellulose mixed cellulose (CA–NC), acetyl cellulose (CA), nylon (JN), polypropylene (PP), nitrocellulose (NC), polyvinylidene fluoride (PVDF), polycarbonate (PC) and polytetrafluoroethylene (PTFE). Physicochemical analysis and mechanical tests indicated that CA, JN and PP membranes yield high adhesivity and reasonable compressive and/or tensile features with friendly surface topography for cell seeding. Cells prefer to adhere on CA, JN, PP or PTFE membranes with high proliferation rate in spheriod-like shape. Actin, albumin and cytokeratin 18 expressions are favorable for cells on CA or PP membrane, whereas protein filtration is consistent among all the eight membranes. These results further the understandings of cell growth, morphology and spreading, as well as protein filtration on distinct membranes in designing a liver bioreactor. |
| 原文出处 | https://doi.org/10.1093/rb/rbu018 |
| 语种 | 英语 |
| 源URL | [http://dspace.imech.ac.cn/handle/311007/60698]  |
| 专题 | 力学研究所_国家微重力实验室 |
| 作者单位 | 中国科学院微重力重点实验室 |
| 推荐引用方式 GB/T 7714 | Lv DY,Gao YX,Luo CH,et al. Selectivity of biopolymer membranes using HepG2 cells[J]. Regenerative Biomaterials,2015,2(1):21-29. |
| APA | Lv DY.,Gao YX.,Luo CH.,Lv SQ.,Wang Q.,...&Long M.(2015).Selectivity of biopolymer membranes using HepG2 cells.Regenerative Biomaterials,2(1),21-29. |
| MLA | Lv DY,et al."Selectivity of biopolymer membranes using HepG2 cells".Regenerative Biomaterials 2.1(2015):21-29. |
入库方式： OAI收割
来源：力学研究所
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