Sensitive, Label-Free Protein Assay Using 1-ethyl-3-methylimidazolium Tetrafluoroborate Supported Microchip Electrophoresis with Laser-Induced Fluorescence Detection
文献类型：期刊论文
| 作者 | Xu YH ; Li J ; Wang EK |
| 刊名 | electrophoresis
 |
| 出版日期 | 2008 |
| 卷号 | 29期号:9页码:1852-1858 |
| 关键词 | INDUCED FLUORESCENCE DETECTION TOTAL ANALYSIS SYSTEMS POLY(DIMETHYLSILOXANE) MICROFLUIDIC DEVICES DODECYL BENZENE SULFONATE ACRIDINE-ORANGE DIMER BETA-D-MALTOSIDE CAPILLARY-ELECTROPHORESIS IONIC LIQUIDS SURFACE MODIFICATION ADSORPTION |
| ISSN号 | 0173-0835 |
| 通讯作者 | wang ek |
| 中文摘要 | based on the dimer-monomer equilibrium movement of the fluorescent dye pyronin y (py), a rapid, simple, highly sensitive, label-free method for protein detection was developed by microchip electrophoresis with lif detection. py formed a nonfluorescent dimer induced by the premicellar aggregation of an anionic surfactant, sds, however, the fluorescence intensity of the system increased dramatically when proteins such as bsa, bovine hemoglobin, cytochrome c, and trypsin were added to the solution due to the transition of dimer to fluorescent monomer. furthermore, 1-ethyl-3-methylimidazolium tetrafluoroborate (emimbf(4)) instead of pbs was applied as running buffers in microchip electrophoresis. |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2010-04-14 ; 2011-06-09 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/10391]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Xu YH,Li J,Wang EK. Sensitive, Label-Free Protein Assay Using 1-ethyl-3-methylimidazolium Tetrafluoroborate Supported Microchip Electrophoresis with Laser-Induced Fluorescence Detection[J]. electrophoresis,2008,29(9):1852-1858. |
| APA | Xu YH,Li J,&Wang EK.(2008).Sensitive, Label-Free Protein Assay Using 1-ethyl-3-methylimidazolium Tetrafluoroborate Supported Microchip Electrophoresis with Laser-Induced Fluorescence Detection.electrophoresis,29(9),1852-1858. |
| MLA | Xu YH,et al."Sensitive, Label-Free Protein Assay Using 1-ethyl-3-methylimidazolium Tetrafluoroborate Supported Microchip Electrophoresis with Laser-Induced Fluorescence Detection".electrophoresis 29.9(2008):1852-1858. |
入库方式： OAI收割
来源：长春应用化学研究所
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