A large scale separation of taxanes from the bark extract of taxus yunnanesis and ~H-and ~13CNMR assignments for 7-epi-10-deacetyltaxol
文献类型：期刊论文
| 作者 | Xue J(薛军) ; Cao CY(曹春阳) ; Chen JM(陈建民) ; Bu HS(卜海山) ; Wu HM(吴厚铭) |
| 刊名 | Chin. J. Chem.
 |
| 出版日期 | 2001 |
| 卷号 | 19期号:1页码:82-90 |
| ISSN号 | 1001-604X |
| 其他题名 | 从云南紫杉提取物中大量分离紫杉烷和7表,10去乙酰紫杉醇的1H-and 13C NMR 归属 |
| 通讯作者 | 陈建民 ; 吴厚铭 |
| 英文摘要 | A large-scale separation of paclitaxel from semi-purified bark extract of taxus yunnanesis was investigated. The chromatographic behavior of paclitaxel and two close eluting analogues, cephalomannine and 7-epi-10-deacetyltaxol were systematically studied on a C^18 bonded phase column with different mobile phase in reverse phase mode. According to the notably different selectivity of the methand and acetonitrile with water in the mobile phase and the most important requirement of capacity in preparative chromatography, the optimum suitablymobile phase used in a large-scale isolation of paclitaxel and 7-epi-10-deacetyltaxol on a preparative C^18 column was give. Cephalomannine was eliminated by ozonolysis and after then separated throughout a normal phase silica column. The whole large-scale process for high purity paclitaxel from the bark extract of Taxus yunnanesis consisted of a preliminary purification with biotage FLASH 150i system based on a prepaked normal phase silica cartridge followed by using a C^18 Nova-pak~TM 4000 preparative HPLC system. The structure of 7-epi-10-deacetyltaxol was elucidated by 2D NMR tecnologies of TOCSY, DQF-COSY, HMQC and HMBC etc. |
| 学科主题 | 天然产物有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1002/cjoc.20010190115 |
| 语种 | 英语 |
| 公开日期 | 2013-02-21 |
| 源URL | [http://202.127.28.38/handle/331003/17285]  |
| 专题 | 上海有机化学研究所_上海有机化学研究所 |
| 推荐引用方式 GB/T 7714 | Xue J,Cao CY,Chen JM,et al. A large scale separation of taxanes from the bark extract of taxus yunnanesis and ~H-and ~13CNMR assignments for 7-epi-10-deacetyltaxol[J]. Chin. J. Chem.,2001,19(1):82-90. |
| APA | 薛军,曹春阳,陈建民,卜海山,&吴厚铭.(2001).A large scale separation of taxanes from the bark extract of taxus yunnanesis and ~H-and ~13CNMR assignments for 7-epi-10-deacetyltaxol.Chin. J. Chem.,19(1),82-90. |
| MLA | 薛军,et al."A large scale separation of taxanes from the bark extract of taxus yunnanesis and ~H-and ~13CNMR assignments for 7-epi-10-deacetyltaxol".Chin. J. Chem. 19.1(2001):82-90. |
入库方式： OAI收割
来源：上海有机化学研究所
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