Cysteine 397 plays important roles in the folding of the neuron-restricted silencer factor/RE1-silencing transcription factor
文献类型：期刊论文
| 作者 | Zhang Y(张岩) ; Hu W(胡伟) ; Shen J(申杰) ; Tong XT(童孝田) ; Yang ZZ(杨中正) ; Chen ZZ(谌章舟) ; Lan WX(蓝文贤) ; Wu HM(吴厚铭) ; Cao CY(曹春阳) |
| 刊名 | Biochem. Biophys. Res. Commun.
 |
| 出版日期 | 2011 |
| 卷号 | 414期号:2页码:309-314 |
| ISSN号 | 0006-291X |
| 其他题名 | 神经元限制性沉默因子397位半胱氨酸在其折叠中发挥重要作用 |
| 通讯作者 | 曹春阳 |
| 英文摘要 | The neuron-restrictive silencer factor/RE1-silencing transcription factor (NRSF/REST) is regarded as not only a key transcriptional repressor but also an activator in neuron gene expression by specifically interacting with neuron-restrictive silencer element (NRSE/RE1) dsDNA and small NRSE/RE1 dsRNA, respectively. But its exact mechanism remains unclear. One major problem is that it is hard to obtain its functional multiple zinc finger (ZnF) domains in a large quantity for further structural studies. To address this issue, in this study, we for the first time attained soluble NRSF/REST functional domains named as ZnF5-8, ZnF4-8, ZnF3-8 and ZnF2-8 containing four, five, six and seven ZnF motifs in tandem, respectively, by using Circular Dichroism (CD) spectrum and two-dimensional (2D) nucleic magnetic resonance (NMR) (1)H-(1)H NOESY spectrum to monitor the folding of each single ZnF peptide. The data indicated that the residue cysteine 397 (Cys397) plays important roles in the global folding of NRSF/REST multiple ZnFs domain. (C) 2011 Elsevier Inc. All rights reserved. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1016/j.bbrc.2011.09.045 |
| 语种 | 英语 |
| WOS记录号 | WOS:000296404800007 |
| 公开日期 | 2013-02-21 |
| 源URL | [http://202.127.28.38/handle/331003/17407]  |
| 专题 | 上海有机化学研究所_上海有机化学研究所 |
| 推荐引用方式 GB/T 7714 | Zhang Y,Hu W,Shen J,et al. Cysteine 397 plays important roles in the folding of the neuron-restricted silencer factor/RE1-silencing transcription factor[J]. Biochem. Biophys. Res. Commun.,2011,414(2):309-314. |
| APA | 张岩.,胡伟.,申杰.,童孝田.,杨中正.,...&曹春阳.(2011).Cysteine 397 plays important roles in the folding of the neuron-restricted silencer factor/RE1-silencing transcription factor.Biochem. Biophys. Res. Commun.,414(2),309-314. |
| MLA | 张岩,et al."Cysteine 397 plays important roles in the folding of the neuron-restricted silencer factor/RE1-silencing transcription factor".Biochem. Biophys. Res. Commun. 414.2(2011):309-314. |
入库方式： OAI收割
来源：上海有机化学研究所
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