Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein
文献类型：期刊论文
| 作者 | Zheng YT(郑永唐) ; Bi KL(贲昆龙) ; Jin SW(金善炜) |
| 刊名 | Acta Pharmacol. Sin.
 |
| 出版日期 | 2000 |
| 卷号 | 21期号:2页码:179-0182 |
| ISSN号 | 1671-4083 |
| 其他题名 | 具有抗HIV-1活性的新核糖体失活蛋白-栝楼蛋白 |
| 通讯作者 | 贲昆龙 |
| 英文摘要 | To determine whether trichobitacin, a novel ribosome-inactivating protein purified from the root tubers of trichosanthes kirlowii, possesses the anti-HIV activity. Methods: the inhibition of syncytial cell formaion induced by human immunodeficiency virus type 1 (HIV-1) was determined under microscope, reduction of HIV-1P24 antigen expression level was measured by ELISA, and decrease in numbers of HIV-1 antigen positive cells in acutely and chronically infected cultures were detected by indirect immunofluorescence assay. Results: Trichobitacin was found to greatly suppres syncytial cell formation induced by HIV-1 and to markedly reduce both expression HIV-1 p24 antigen and the number of HIV-1antigen positive cells in acutely but not chronically HIV-1 infected culture. The median inhibitory concentration (IC^50) in inhibition of syncytial cell formation and HIV antigen positive cells were 5 μg.L-1(95 % confidence limits: 1,3-20 μg.L-1) and 0.09 mg.L-~1(95% confidence limits: 0.011-0.755 mg.L~1), respectively. Conclusion: Trichobitacin is a novel ribosome-inactivating protein with anti-HIV-1activity. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000085208200014 |
| 公开日期 | 2013-03-12 |
| 源URL | [http://202.127.28.38/handle/331003/24711]  |
| 专题 | 上海有机化学研究所_上海有机化学研究所 |
| 推荐引用方式 GB/T 7714 | Zheng YT,Bi KL,Jin SW. Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein[J]. Acta Pharmacol. Sin.,2000,21(2):179-0182. |
| APA | 郑永唐,贲昆龙,&金善炜.(2000).Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein.Acta Pharmacol. Sin.,21(2),179-0182. |
| MLA | 郑永唐,et al."Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein".Acta Pharmacol. Sin. 21.2(2000):179-0182. |
入库方式： OAI收割
来源：上海有机化学研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。