A Novel Small Molecule Regulator of Guanine Nucleotide Exchange Activity of the ADP-ribosylation Factor and Golgi Membrane Trafficking
文献类型：期刊论文
| 作者 | Pan HL(潘鹤龄) ; Yu J(俞佳) ; Zhang LH(张立红) ; Carpenter, Anne ; Zhu, Hong ; Li L(李莉) ; Ma DW(马大为) ; Yuan JY(袁钧英) |
| 刊名 | J. Biol. Chem.
 |
| 出版日期 | 2008 |
| 卷号 | 283期号:45页码:31087-31096 |
| ISSN号 | 0021-9258 |
| 其他题名 | A Novel Small Molecule Regulator of Guanine Nucleotide Exchange Activity of the ADP-ribosylation Factor and Golgi Membrane Trafficking |
| 通讯作者 | 马大为 ; 袁钧英 |
| 英文摘要 | An image-based phenotypic screen was developed to identify small molecule regulators of intracellular traffic. Using this screen we found that AG1478, a previously known inhibitor of epidermal growth factor receptor, had epidermal growth factor receptor-independent activity in inducing the disassembly of the Golgi in human cells. Similar to brefeldin A (BFA), a known disrupter of the Golgi, AG1478 inhibits the activity of small GTPase ADP-ribosylation factor. Unlike BFA, AG1478 exhibits low cytotoxicity and selectively targets the cis-Golgi without affecting endosomal compartment. We show that AG1478 inhibits GBF1, a large nucleotide exchange factor for the ADP-ribosylation factor, in a Sec7 domain-dependent manner and mimics the phenotype of a GBF1 mutant that has an inactive mutation. The treatment with AG1478 leads to the recruitment of GBF1 to the vesicular-tubular clusters adjacent to the endoplasmic reticulum exit sites, a step only transiently observed previously in the presence of BFA. We propose that the treatment with AG1478 delineates a membrane trafficking intermediate step that depends upon the Sec7 domain. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1074/jbc.M806592200 |
| 语种 | 英语 |
| WOS记录号 | WOS:000260544800063 |
| 公开日期 | 2013-02-19 |
| 源URL | [http://202.127.28.38/handle/331003/15565]  |
| 专题 | 上海有机化学研究所_生命有机化学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Pan HL,Yu J,Zhang LH,et al. A Novel Small Molecule Regulator of Guanine Nucleotide Exchange Activity of the ADP-ribosylation Factor and Golgi Membrane Trafficking[J]. J. Biol. Chem.,2008,283(45):31087-31096. |
| APA | 潘鹤龄.,俞佳.,张立红.,Carpenter, Anne.,Zhu, Hong.,...&袁钧英.(2008).A Novel Small Molecule Regulator of Guanine Nucleotide Exchange Activity of the ADP-ribosylation Factor and Golgi Membrane Trafficking.J. Biol. Chem.,283(45),31087-31096. |
| MLA | 潘鹤龄,et al."A Novel Small Molecule Regulator of Guanine Nucleotide Exchange Activity of the ADP-ribosylation Factor and Golgi Membrane Trafficking".J. Biol. Chem. 283.45(2008):31087-31096. |
入库方式： OAI收割
来源：上海有机化学研究所
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