Syntheses and characterizations of novel pyrrolocoumarin probes for SNAP-tag labeling technology
文献类型：期刊论文
| 作者 | Mei DS(梅德盛) ; QU YI ; HE JINXIANG ; Chen L(陈磊) ; Yao ZJ(姚祝军) |
| 刊名 | Tetrahedron
 |
| 出版日期 | 2011 |
| 卷号 | 67期号:12页码:2251-2259 |
| ISSN号 | 0040-4020 |
| 其他题名 | 用于融合蛋白荧光标记技术的新型吡咯香豆素类探针的合成及表征 |
| 通讯作者 | 姚祝军 |
| 英文摘要 | SNAP-tag technology is a revolutionary protein labeling technology employing in various biological studies. Since low signal/noise ratio and severe overlap between the FRET donors/acceptors often occurred in applying present fluorescent probes and thus limited the further applications, development of new fluorescent probes with excellent fluorescent properties is still of request by today's SNAP-tag technology. In this paper, a number of SNAP-tag protein probes have been developed by incorporating a novel pyrrolocoumarin fluorophore recently developed by our group. Examination of these novel synthetic compounds shows all these materials possess satisfactory fluorescent properties. Among these, probe 7 exhibits the most excellent characters, and its quantum yield, maximum emission wavelength and Stocks shift reach to 0.44, 534 nm and 112 nm, respectively. Further analysis of structure-property relationship indicates that the probes with a longer C3-substituted alkyl (such as pentyl) give stronger fluorescence. (C) 2011 Elsevier Ltd. All rights reserved. |
| 学科主题 | 生命有机化学 |
| 收录类别 | SCI |
| 原文出处 | http://dx.doi.org/10.1016/j.tet.2011.01.075 |
| 语种 | 英语 |
| WOS记录号 | WOS:000288778100009 |
| 公开日期 | 2013-02-26 |
| 源URL | [http://202.127.28.38/handle/331003/21149]  |
| 专题 | 上海有机化学研究所_生命有机化学国家重点实验室 |
| 推荐引用方式 GB/T 7714 | Mei DS,QU YI,HE JINXIANG,et al. Syntheses and characterizations of novel pyrrolocoumarin probes for SNAP-tag labeling technology[J]. Tetrahedron,2011,67(12):2251-2259. |
| APA | 梅德盛,QU YI,HE JINXIANG,陈磊,&姚祝军.(2011).Syntheses and characterizations of novel pyrrolocoumarin probes for SNAP-tag labeling technology.Tetrahedron,67(12),2251-2259. |
| MLA | 梅德盛,et al."Syntheses and characterizations of novel pyrrolocoumarin probes for SNAP-tag labeling technology".Tetrahedron 67.12(2011):2251-2259. |
入库方式： OAI收割
来源：上海有机化学研究所
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