Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana
文献类型：期刊论文
| 作者 | Wang, J; Ying, SH; Hu, Y; Feng, MG |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 101期号:1页码:185-195 |
| 关键词 | Entomopathogenic fungi J-domain proteins Asexual development Cell wall integrity Multiple stress tolerance Biological control potential |
| 通讯作者 | mgfeng@zju.edu.cn |
| 中文摘要 | Mdj1 is a member of the Hsp40 family containing a DnaJ or J domain. Here, we have examined the functions of an Mdj1 orthologue (56.68 kDa) in Beauveria bassiana, a filamentous fungal insect pathogen widely applied in biological control of insect pests. Deletion of mdj1 in B. bassiana resulted in significant growth defects on a variety of complex and minimal media. The Delta mdj1 mutant exhibited not only a drastic reduction (92 %) in aerial conidiation during optimal cultivation but also a remarkable decrease (77 %) in submerged blastospore production. Compared to wild-type, the deletion mutant was significantly more sensitive to the stresses of cell wall perturbation, high osmolarity, oxidation, carbendazim fungicide, several metal ions, and acidic/alkaline pH during conidial germination and/or colony growth. In the mutant, conidial thermotolerance and UV-B resistance decreased by 61 and 25 %, respectively, and virulence to Galleria mellonella larvae was largely attenuated. Transcript levels of many phenotype-related genes were drastically suppressed in the absence of mdj1, accompanied with impaired cell walls and reduced intracellular anti-stress molecules, including superoxide dismutases, catalases, glycerol, trehalose, and mannitol. These data indicate that Mdj1 plays a vital role in normal fungal development and contributes significantly to the biological control potential of B. bassiana against insect pests. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/16210]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Wang, J,Ying, SH,Hu, Y,et al. Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2017,101(1):185-195. |
| APA | Wang, J,Ying, SH,Hu, Y,&Feng, MG.(2017).Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,101(1),185-195. |
| MLA | Wang, J,et al."Vital role for the J-domain protein Mdj1 in asexual development, multiple stress tolerance, and virulence of Beauveria bassiana".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 101.1(2017):185-195. |
入库方式： OAI收割
来源：南海海洋研究所
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