High Efficiency Hydrodynamic DNA Fragmentation in a Bubbling System
文献类型：期刊论文
| 作者 | Li, LH; Jin, ML; Sun, CL; Wang, XX; Xie, ST; Zhou, GF; Van den Berg, A; Eijkel, JCT; Shui, LL |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017 |
| 卷号 | 7页码:40745- |
| 通讯作者 | shuill@m.scnu.edu.cn |
| 中文摘要 | DNA fragmentation down to a precise fragment size is important for biomedical applications, disease determination, gene therapy and shotgun sequencing. In this work, a cheap, easy to operate and high efficiency DNA fragmentation method is demonstrated based on hydrodynamic shearing in a bubbling system. We expect that hydrodynamic forces generated during the bubbling process shear the DNA molecules, extending and breaking them at the points where shearing forces are larger than the strength of the phosphate backbone. Factors of applied pressure, bubbling time and temperature have been investigated. Genomic DNA could be fragmented down to controllable 1-10 Kbp fragment lengths with a yield of 75.30-91.60%. We demonstrate that the ends of the genomic DNAs generated from hydrodynamic shearing can be ligated by T4 ligase and the fragmented DNAs can be used as templates for polymerase chain reaction. Therefore, in the bubbling system, DNAs could be hydrodynamically sheared to achieve smaller pieces in dsDNAs available for further processes. It could potentially serve as a DNA sample pretreatment technique in the future. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/16230]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Li, LH,Jin, ML,Sun, CL,et al. High Efficiency Hydrodynamic DNA Fragmentation in a Bubbling System[J]. SCIENTIFIC REPORTS,2017,7:40745-. |
| APA | Li, LH.,Jin, ML.,Sun, CL.,Wang, XX.,Xie, ST.,...&Shui, LL.(2017).High Efficiency Hydrodynamic DNA Fragmentation in a Bubbling System.SCIENTIFIC REPORTS,7,40745-. |
| MLA | Li, LH,et al."High Efficiency Hydrodynamic DNA Fragmentation in a Bubbling System".SCIENTIFIC REPORTS 7(2017):40745-. |
入库方式： OAI收割
来源：南海海洋研究所
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