c-Jun N-terminal kinase (JNK) is involved in immune defense against bacterial infection in Crassostrea hongkongensis
文献类型：期刊论文
| 作者 | Qu, FF; Xiang, ZM; Xiao, S; Wang, FX; Li, J; Zhang, Y; Zhang, YH; Qin, YP; Yu, ZN |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 67页码:77-85 |
| 关键词 | c-Jun N-terminal kinase Molecular characteristics Expression analysis Bacterial challenge C. hongkongensis |
| 通讯作者 | zhimingxiang@scsio.ac.cn ; carlzyu@scsio.ac.cn |
| 中文摘要 | c-Jun N-terminal kinase (JNK) is a universal and essential subgroup of the mitogen-activated protein kinase (MAPK) superfamily, which is highly conserved from yeast to mammals and functions in a variety of physiological and pathological processes. In this study, we report the first oyster JNK gene homolog (ChJNK) and its biological functions in the Hong Kong oyster Crassostrea hongkongensis. The ChJNK protein consists of 383 amino acids and contains a conserved serine/threonine protein kinase (S_TKc) domain with a typical TPY motif. Phylogenetic analysis revealed that ChJNK shared a close evolutionary relationship with Crassostrea gigas JNK. Quantitative RT-PCR analyses revealed broad expression patterns of ChJNK mRNA in various adult tissues and different embryonic and larval stages of C hongkongensis. When exposed to Vibrio alginolyticus or Staphylococcus haemolyticus, ChJNK mRNA expression levels were significantly up-regulated in the hemocytes and gills in a time-dependent manner. Additionally, sub cellular localization studies that ChJNK is a cytoplasm-localized protein, and that its overexpression could significantly enhance the transcriptional activities of AP-1-Luc in HEK293T cells. In summary, this study provided the first experimental demonstration that oysters possess a functional JNK that participates in host defense against bacterial infection in C. hongkongensis. (C) 2016 Elsevier Ltd. All rights reserved. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/16231]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Qu, FF,Xiang, ZM,Xiao, S,et al. c-Jun N-terminal kinase (JNK) is involved in immune defense against bacterial infection in Crassostrea hongkongensis[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2017,67:77-85. |
| APA | Qu, FF.,Xiang, ZM.,Xiao, S.,Wang, FX.,Li, J.,...&Yu, ZN.(2017).c-Jun N-terminal kinase (JNK) is involved in immune defense against bacterial infection in Crassostrea hongkongensis.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,67,77-85. |
| MLA | Qu, FF,et al."c-Jun N-terminal kinase (JNK) is involved in immune defense against bacterial infection in Crassostrea hongkongensis".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 67(2017):77-85. |
入库方式： OAI收割
来源：南海海洋研究所
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