A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro
文献类型：期刊论文
| 作者 | Qu, FF; Xiang, ZM; Li, J; Xiao, S; Mao, F; Qin, YP; Zhou, YL; Ma, HT; Yu, ZN |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2017 |
| 卷号 | 62页码:311-319 |
| 关键词 | Extracellular signal-regulated kinase Expression patterns Immune challenge Subcellular localization Crassostrea hongkongensis |
| 通讯作者 | zhimingxiang@scsio.ac.cn ; carlzyu@scsio.ac.cn |
| 中文摘要 | Extracellular signal -regulated kinases (ERKs) are a group of highly conserved serine/threonine-specific protein kinases that function as important signaling intermediates in mitogen-activated protein kinase (MAPK) pathways, which are involved in a wide variety of cellular activities, including proliferation, inflammation and cytokine production. However, little is known about the roles of this kinase in mollusk immunity. In this study, we identified a molluscan ERK homolog (ChERK) in the Hong Kong oyster (Crassostrea hongkongensis) and investigated its biological functions. The open reading frame (ORF) of ChERK encoded a polypeptide of 365 amino acids, with a predicted molecular weight of 41.96 kDa and pI of 6.43. The predicted ChERK protein contained typical characteristic motifs of the ERK family, including a dual threonine-glutamate-tyrosine (TEY) phosphorylation motif and an ATRW substrate binding site. Phylogenetic analysis revealed that ChERK belonged to the mollusk cluster and shared a close evolutionary relationship with ERIC from Crassostrea gigas. In addition, quantitative real-time PCR analysis revealed that ChERK expression was detected in all of the examined tissues and stages of embryonic development; its transcript level was significantly induced upon challenge with bacterial pathogens (Vibrio alginolyticus and Staphylococcus haemolyticus) in vivo and PAMPs (lipopolysaccharide and peptidoglycan) in vitro. Moreover, ChERK was mainly located in the cytoplasm of HEIC293T cells. Taken together, these findings may provide novel insights into the functions of molluscan ERKs, especially their roles in response to immune challenge in oyster. (C) 2017 Published by Elsevier Ltd. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/16244]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Qu, FF,Xiang, ZM,Li, J,et al. A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro[J]. FISH & SHELLFISH IMMUNOLOGY,2017,62:311-319. |
| APA | Qu, FF.,Xiang, ZM.,Li, J.,Xiao, S.,Mao, F.,...&Yu, ZN.(2017).A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro.FISH & SHELLFISH IMMUNOLOGY,62,311-319. |
| MLA | Qu, FF,et al."A molluscan extracellular signal-regulated kinase is involved in host response to immune challenges in vivo and in vitro".FISH & SHELLFISH IMMUNOLOGY 62(2017):311-319. |
入库方式： OAI收割
来源：南海海洋研究所
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