Establishment and characterization of a mid-kidney cell line derived from golden pompano Trachinotus ovatus, a new cell model for virus pathogenesis and toxicology studies
文献类型：期刊论文
| 作者 | Zhou, LL; Li, PF; Liu, JX; Ni, SW; Yu, YP; Yang, M; Wei, SN; Qin, QW |
| 刊名 | IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
 |
| 出版日期 | 2017 |
| 卷号 | 53期号:4页码:320-327 |
| 关键词 | Trachinotus ovatus Mid-kidney cell line Cytotoxicity Nervous necrosis virus Heavy metals |
| 通讯作者 | weisn@scsio.ac.cn ; qinqiwei64@outlook.com |
| 中文摘要 | Golden pompano Trachinotus ovatus, a popularly cultured and commercially important marine fish worldwide, has been recognized as a promising candidate for mariculture. However, outbreaks of infectious bacterial or viral diseases and environmental deterioration have led to great economic losses in T. ovatus aquaculture recently. In our research, we established a new mid-kidney cell line, designated as TOK, from golden pompano, T. ovatus. The optimized growth temperature and working concentration of fetal bovine serum (FBS) were 28A degrees C and 10-20%, respectively. Foreign genes could express well in TOK cells. The modal number of TOK cells was 54. The TOK cells were susceptive to Singapore grouper iridovirus (SGIV) and red-spotted grouper nervous necrosis virus (RGNNV), and the virus could propagate in cells. Propagation was verified by qRT-PCR, and virions were observed under electron microscopy. Cytotoxicity analysis revealed that TOK cells were sensitive to different concentrations of extracellular products (ECPs) from Vibrio alginolyticus and V. anguillarum. Moreover, heavy metals (Cd, Cu, and Hg) also showed dose-dependent cytotoxicity to the TOK cell line. We established a mid-kidney cell line from T. ovatus which could be applied to cytotoxicity assays of heavy metals. The newly established TOK cell line possesses great application potential in genetic manipulation, virus-host interaction studies, and toxicity assays of bacterial extracellular products and heavy metals. |
| 源URL | [http://ir.scsio.ac.cn/handle/344004/16259]  |
| 专题 | 南海海洋研究所_中科院海洋生物资源可持续利用重点实验室 |
| 推荐引用方式 GB/T 7714 | Zhou, LL,Li, PF,Liu, JX,et al. Establishment and characterization of a mid-kidney cell line derived from golden pompano Trachinotus ovatus, a new cell model for virus pathogenesis and toxicology studies[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,2017,53(4):320-327. |
| APA | Zhou, LL.,Li, PF.,Liu, JX.,Ni, SW.,Yu, YP.,...&Qin, QW.(2017).Establishment and characterization of a mid-kidney cell line derived from golden pompano Trachinotus ovatus, a new cell model for virus pathogenesis and toxicology studies.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL,53(4),320-327. |
| MLA | Zhou, LL,et al."Establishment and characterization of a mid-kidney cell line derived from golden pompano Trachinotus ovatus, a new cell model for virus pathogenesis and toxicology studies".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL 53.4(2017):320-327. |
入库方式： OAI收割
来源：南海海洋研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。